3种抗原Dot-ELISA诊断日本血吸虫病的比较  

Comparison of Three Antigens in the Diagnosis of Schistosomiastis japonicum by the Dot ELISA

作　　者：沈定文[1] 罗金萍[1] 陈喜圭[1] 

机构地区：[1]湖北咸宁医学院寄生虫学教研室,437100

出　　处：《中国公共卫生》2001年第5期446-447,共2页Chinese Journal of Public Health

基　　金：湖北省教委重点课题资助!(94A0 63)

摘　　要：目的　探讨Dot-ELISA快速诊断血吸虫病的实用价值。方法　利用Dot ELISA分别检测 10 8例日本血吸虫病人、2 5例肝吸虫病人、2 0例肺吸虫病和 5 0例正常人血清日本血吸虫 31/ 32kDa抗原 (Sj31/ 32 )、可溶性虫卵抗原 (SEA)和血吸虫成虫粗抗原 (AWA)特异性IgG抗体 ,以寻找用于Dot ELISA快速有效诊断日本血吸虫病的抗原。结果　 10 8例日本血吸虫病人血清Sj31/ 32 IgG、SEA IgG和AWA IgG的检出率分别为 92 6 %、94 4%和 95 4% ,平均抗体滴度分别为 1∶333、1∶982和 1∶85 0 ,5 0例正常人和 2 5例肝吸虫病人Sj31/ 32 IgG均为阴性 ,2 0例肺吸虫病人中Sj31/ 32 IgG仅 1例阳性 ,而正常人、肝吸虫和肺吸虫病人SEA IgG、AWA IgG均有不同程度的交叉反应。Sj31/ 32 IgG与SEA、AWA敏感性相似 ,但特异性明显高于SEA和AWA。结论　提示Sj31/ 32 IgGDot ELISA诊断日本血吸虫病价值优于SEA和AWA。Objectives To find an effective antigen applied in the diagnosis of schistosomiasis japonicum.Methods Dot ELISA was used in the detection of Schistosoma japonicum 31/32kDa antigen(Sj31/32) IgG、soluble egg antigen(SEA) IgG and adult worm antigen(AWA)IgG in 108 cases of patient with schistosomiasis、25 cases of patient with Clonorchiasis、20 cases of patient with Paragonimiasis and 50 cases of normal human.Results The positive rates of Sj31/32 IgG、SEA IgG and AWA IgG were 92 6%、94 4% and 95 4% respectivity in 108 case of schistosomiasis,while the mean antibody titers were 1∶333、1∶982 and 1∶850 respectivity.The Sj31/32 IgG was negative in sera of Clonorchiasis and normal person.Only one case was positive in the 20 cases of Paragonimiasis.Some nonspecific reaction were emerged by SEA and AWA with sera of normal human、Paragonimiasis and Clonorchiasis.The sensitivity of Sj31/32 IgG was similar with the SEA and AWA,but the specificity was higher than that of SEA and AWA.Conclusion The results indicated that the value of Sj31/32 IgG in the diagnosis of schistosomiasis japonicum by Dot ELISA might be superior to that of SEA and AWA.

关 键 词：日本血吸虫病 DOT-ELISA Sj31/32 AWA SEA 

分 类 号：R532.21[医药卫生—内科学]