荧光光度法测定大豆提取液中还原型谷胱甘肽  

Determination of the reduced glutathione by fluorescence spectrophotometry

作　　者：郭黎平[1] 刘国良[1] 张卓勇[1] 刘思东[1] 王丽颖[2] 

机构地区：[1]东北师范大学化学学院,吉林长春130024 [2]白求恩医科大学基础医学院,吉林长春130021

出　　处：《东北师大学报（自然科学版）》2001年第1期34-38,共5页Journal of Northeast Normal University(Natural Science Edition)

基　　金：吉林省卫生厅科研基金

摘　　要：在Cohn的荧光分光光度法的基础上 ,不加去蛋白试剂 ,用 365nm作为激发波长 ,探讨了用荧光光度法测定大豆提取液中的还原型谷胱甘肽的方法 ,确定了最佳测定条件 .该方法用于测定大豆提取液中谷胱甘肽的含量 ,取得了满意结果 .An improved fluorescence spectrophotometric method was developed for the reduced glutathione(GSH) determination based on the reaction of GSH with orthophthaldehyde (OPA). Some affecting parameters including amount of OPA, stability of the fluorescence system, etc. were investigated and optimized. Linear dynamic relationship was obtained in the concentration range of 0.08～0.8 μg/mL for glutathione. The regression equation was as following: I=0.984 4+84.15c . The correlation coefficient was 0.999 9. The method was also applied to determine GSH in soybean extracts by electromagnetic field technique and satisfactory results were obtained.

关 键 词：还原型谷胱甘肽 大豆提取液 荧光光度法 测定 

分 类 号：Q516[生物学—生物化学] O657.31[理学—分析化学]