借铝(Ⅲ)螯合物和高效液相色谱测定四环素类药物(英文)  

Determination of Tetracyclines as Chelates With Aluminium(Ⅲ) by HPLC With Fluorescence Detection

作　　者：张孝松[1] 刘轶春[1] 王蕾[1] 林长山[1] 

机构地区：[1]中国科学技术大学化学系,合肥230026

出　　处：《中国科学技术大学学报》2001年第2期213-217,共5页JUSTC

摘　　要：借离子对反相高效液相色谱分离美满霉素 (MNC)、土霉素 (OTC)、美他霉素(MTC)和金霉素 (CTC)的铝 (Ⅲ )螯合物并于激发波长 380nm和发射波长 480nm处检测 研究了螯合物的最宜分离和测定条件 于KromasilODS柱 (2 5 0× 4 6mmI D ,5 μm)上 ,用含 0 0 5 0mol/L柠檬酸缓冲剂 (pH 2 5 )作流动相 ,流速为 0 7mL/min 检测限为 0 1 2ngMNC ,0 2ngOTC ,1 8ngMTC和 0Minocycline(MNC), oxytetracycline(OTC), methacycline(MTC) and chlortetracycline(CTC) as chelates derivatized pre-column in a solution of 0 4 mol/L acetic acid-sodium acetate buffer (pH 4 0) with aluminium(Ⅲ) were separated by ion-pair reversed-phase high-performance liquid chromatography and detected spectrofluorimetrically with excitation wavelength at 380 nm and emssion wavelength at 480 nm The optimum conditions for the separation and determination of MNC-, OTC-, MTC- and CTC-Al(Ⅲ) chelates were investigated The chelates were separated on a Kromasil ODS column (250×4 6 mm I D , 5 μm) using acetonitrile-dimethylformamide-water (5∶20∶75, V/V/V) containing 0 050 mol/L citric acid buffer (pH 2 5) as a mobile phase at a flow rate of 0 7 mL/min The detection limits of the assay (signal-to-noise ratio = 3∶1) were 0 12 ng, 0 2 ng, 1 8 ng and 0 6 ng for MNC, OTC, MTC and CTC, respectively

关 键 词：液相色谱 四环素类药物 衍生剂Ⅲ 荧光检测 测定 螯合物 

分 类 号：R978.14[医药卫生—药品]