影响腹膜炎细菌培养结果的因素探讨  

Factors influencing recovery of microbial from dialysis effluents

作　　者：汪涛[1] 叶任高[1] 何柏林[1] 陈永雄[1] 李惠群[1] 

机构地区：[1]广州中山医科大学肾脏研究所

出　　处：《肾脏病与透析肾移植杂志》1994年第1期34-36,共3页Chinese Journal of Nephrology,Dialysis & Transplantation

摘　　要：采用透出液在体外和细菌（表皮葡萄球菌和大肠杆菌）一起孵育的方法，以观察透析液在不同条件下对细菌生长的影响。发现在４℃条件下，透出液对细菌生长的影响不大，但在３７℃条件下，透出液中大肠杆菌数则显著减少（Ｐ＜０．０５）；当用细胞冻融破坏透出液中的细胞后，表皮葡萄球菌和大肠杆菌数均显著增加（Ｐ＜０．０１），特别是在含５％ＣＯ２时，新鲜透析液虽支持大肠杆菌的生长，但却抑制表皮葡萄球菌的繁殖。本研究结果提示，透出液中细菌的生长受温度、ＣＯ２、透出液中吞噬细胞及透析液在体内停留的时间等因素影响，特别是透出液中的吞噬细胞。若破坏透出液中的吞噬细胞，有助于提高透出液的细菌培养阳性率。he effect of dialysis on recovery of bacteria under different conditions was studied by incubating dialysis with S.epidermidis(SE)and E.coli(EC)in vitro.Dialysis didn't affect the number of bacteria in 4℃,but significantly decreased the number of EC in 37℃.If the phagocytes in effluent was disrupted by freeze thaw cycle,the number of SE and EC was significantly increased,especially incubated with 5% CO2.Although fresh dialysate supported the growth of EC ,it suppressed the growth of SE.It suggests that the field of bacteria from dialysis effluents is influenced by temperature,CO2, phagocytes in effluents and the dwell time of dialysis and so on.The phagocytes are the most important.Disrupting the phagocytes in dialysis effluents may increase the field of positive culture results.

关 键 词：腹膜透析 透出液 细菌学 细菌性腹膜炎 

分 类 号：R459.5[医药卫生—治疗学]