高纯度线粒体DNA的快速提取  

A Rapid and High Purity Method for Isolation of Mithochondrial DNA

作　　者：梅玉屏[1] 傅先元[2] 叶成仁[2] 

机构地区：[1]广东省中医院中心试验室,广东广州510120 [2]广州师范学院生物系,广东广州510405

出　　处：《广州师院学报（自然科学版）》2000年第3期70-73,共4页

摘　　要：报道了一种改进的线粒体 ＤＮＡ（ ｍｉｔｏｃｈｏｎｄｒｉａｌ ＤＮＡ， ｍｔＤＮＡ）提取方法，该方法不仅使提取ｍｔＤＮＡ的周期缩短了，而且操作简便，提取的线粒体ＤＮＡ结构完整，并彻底避免了核ＤＮＡ、线粒体 ＲＮＡ、蛋白质、有机溶剂等的污染；该方法还可以根据不同的试验要求而选取相应的步骤。与其他方法相比较，该方法具有快速、简便、经济、产品纯度高以及ｍｔＤＮＡ结构完整等特点，其得率和纯度均能满足ｍｔＤＮＡ的各种分析要求。Mitochondrial DNA is widely used in the studying field of mechanism of disease, heredity and breeding of animals, evolution of creatures, and many valuable results have been obtained. The premise is effective methods of mtDNA isolation and purification. Some methods have been adopted such as density gradient centrifugation, enzyme digestion, high speed centrifugation, column chromatography and Alkaline Lysis. A method is reported in this paper, which can completely avoide the possible pollution by nuclear DNA, mtRNA, protein etc. In isolation and purification of mtDNA, some procedures can be omited according to requirement of experiments. This method is quicker and simpler than other methods, and the product is purer. The quality and purity of mtDNA by this method are suitable to all kinds of studies on mtDNA.

关 键 词：线粒体DNA DNASEI 碱变性方法 提取方法 核DNA 线粒体RNA 蛋白质 

分 类 号：Q781[生物学—分子生物学]