乙型肝炎动物模型的研究  

Studies on Animal model for HBV

作　　者：王树声[1] 黄果勇[1] 张涛[1] 葛宪民[1] 苏建家 李媛 覃柳亮 冯百芳[3] 高建恩[3] 张华远[4] 王佑春[4] 

机构地区：[1]广西壮族自治区卫生防疫站,南宁530021 [2]广西壮族自治区肿瘤研究所,南宁530021 [3]北京医科大学肝病研究所,南宁530021 [4]中国药品生物制品检定所,南宁530021

出　　处：《医学研究通讯》2001年第5期7-10,共4页Bulletin of Medical Research

基　　金：国家"九五"科技攻关课题

摘　　要：目的建立树鼩和熊猴的乙肝动物模型。方法用含HBV的人血清接种给7批30只熊猴,6批233只树鼩,每周定期采血、定期肝组织活检。用改良赖氏法、ELISA、PCR、原位杂交、免疫组化分别检测血清ALT、HBV标志物、HBV-DNA及肝组织中HBsAg和HBV-DNA。结果树鼩接种人HBV(HHBV)后,90％呈急性感染,56％为可持续1年以上的慢性感染,33％持续2年2月,肝细胞内HBsAg阳性率达80％,熊猴接种HHBV后,血清HBV标志物呈一过性,个别猴肝细胞内HBsAg呈弱阳性。结论熊猴对HHBV并不敏感,而树鼩对HHBV感染有较高的敏感性,有望成为HHBV的动物模型。Objective To establish animal model for HBV research with tree shrew and macaca assamensis. Methods 233 tree shrews and 30 macaca assamensis were inoculated with human sera containing hepatitis B virus (HBV) .After that, the sera were collected weekly from them and HBV markers were detected with ELISA kits; HBsAg and HBVDNA in hepatic cells were detected with immunohistrochemistry and in situ hybridization. Results Showed that 90% of tree shrews became acute infection, among them, 56% persisted for over one year, 33% of them were chronic infection with persistence of 2 years and 2 months.The positive ratio of HBsAg in hepatic cells is 80% ; the persistence of HBV in macaca assamensis was much shorter. Conclusion The data indicated that tree shrews is a more practical animal model for chronic HBV infection with persistence of viral replication, which is more susceptible than macaca assamensis.

关 键 词：动物模型 乙型肝炎 树QU 熊猴 研究 

分 类 号：R512.62[医药卫生—内科学]