DNA去甲基化引起人二倍体成纤维细胞端区缩短并加速衰老进程  

DNA demethylation leads to telomere shortening with accelerated aging process in human diploid fibroblasts

作　　者：陈培利[1] 童坦君[1] 张宗玉[1] 

机构地区：[1]北京大学基础医学院生物化学与分子生物学系,北京100083

出　　处：《北京大学学报（医学版）》2001年第1期42-45,共4页Journal of Peking University:Health Sciences

基　　金：国家自然科学基金重点项目! ( 39930 170 )&&

摘　　要：目的 :研究DNA甲基化水平变化对细胞衰老进程和端区长度的影响。方法 :从细胞形态和Southern印迹杂交测定端区长度两方面观察 5 氮胞苷处理对人胚肺二倍体成纤维细胞衰老进程的影响。结果 :5 氮胞苷处理使老年细胞衰老表型更为突出 ,其端区长度缩短较年轻细胞亦为显著 (约为对照细胞的 96 % )。结论Objective: The effect of DNA demethylation on aging process and telomere length was studied. Methods: The effect of 5 aza cytidine treatment on aging process of human fetal lung diploid fibroblasts (2BS) was determined by cellular morphology and telomere length assayed by Southern blotting. Results: The results showed that DNA demethylation could advance the senescent phenotype more significantly in old cells than it did in young cells. Accordingly, telomere shortening seemed to be more apparent in old cells (about 96% of that of control). Conclusion: The decreased level of DNA methylation and the resulted telomere shortening may be important cooperative factors contributing to cellular aging process.

关 键 词：去甲基化 DNA 端粒 药物作用 成纤维细胞 细胞衰老 

分 类 号：Q251[生物学—细胞生物学]