水稻转基因再生植株及其后代抗虫性筛选测定研究  

The Selection and Determinaton of the Pest-resistant Character in Transgenic Regenerative Rice Plants and Their Descendances

作　　者：冉学忠[1] 王立敏[1] 孙伯铮[1] 陈方江 刘春颖[1] 

机构地区：[1]铁岭市农业科学院,辽宁铁岭112616

出　　处：《辽宁农业科学》2001年第2期15-18,共4页Liaoning Agricultural Sciences

摘　　要：采用基因枪转化系统，将ＣｐＴＩ（豇豆胰蛋白酶抑制剂）抗虫基因转入水稻愈伤组织中．经过抗生素筛选 得到了再生植株。我们用这些转基因水稻再生植株及其后代的分蘖茎段对玉米螟（Ｏｓｔｒｉｎｉａ　ｎｕｂｉｌａｌｉｓ）进行室 内喂养，通过比较虫体死亡率及虫体生长长度，测定转基因水稻再生植株的抗虫性。１９９７－１９９９年共进行３ 个世代５次的室内测定。从１２１株Ｆ０代转基因植株中选出抗虫性较好的植株１５株。从Ｆ；代的１５个株系 中，又筛选出７个株系４０个单株；在Ｆ２代的１４个株系中共选出３７株抗虫效果表现较好的植珠。对３７株中 的１９株进行了分子检测（ＰＣＲ扩增），从中检测出５份材料为纯合体，１４份材料有分离现象。研究结果说明： 该室内喂养玉米螟并进行抗虫测定的方法是可行的；抗虫基因在转基因水稻再生植株中已经获得表达并且 可以遗传。Using gene gun transformation system, we transformation CpTI (Cowpea Trypsin Inhibitor) gene to rice callus tissues; through anti-biotic selection. The transgenic regenerative rice plants were gotten. Indoor feeding corn borer( Ostrinia nubilalis) by these transgenic rice regenaration plants and their descendance' s tiller stem ; The corn borer mortality and the worm length were compared to determinate transgenic regenerative rice's pest resistance. From 1997 to 1999,5 indoor tests had been taken on three generations. 15 better pest resistance plants were selected from 121 plants in F0 generation, 7 strains 40 better single plants from 15 strains in F1 generation, and 37 better plants from 14 strains in F2 generation. We did molecular test(PCR) to 19 plants from the 37 better plants, and asserted that 5 material are homozygous plants, the other 14 material have segregation. All of these stated that the anti-pest gene in transgenic regenerative rice can express and is heritable.

关 键 词：水稻 转基因 再生植株 玉米螟 抗虫性 筛选 

分 类 号：S511.034[农业科学—作物学]