青霉素裂解液的高效液相色谱分析  

Determination of Degraded Products of Penicillin by High Performance Liquid Chromatography

作　　者：赵静玫[1] 都绛瑛[1] 张铭俊[1] 

机构地区：[1]中国科学院大连化学物理研究所,辽宁大连116023

出　　处：《色谱》2001年第1期88-90,共3页Chinese Journal of Chromatography

基　　金：国家"九.五"重点攻关课题! (96 0C0 2 0 2 0 1)

摘　　要：利用高效液相色谱法分析测定青霉素裂解液中的主要成分 6 氨基青霉烷酸 (6 APA)、青霉素G钾和苯乙酸 ,并对副产物青霉噻唑酸进行了液相色谱的定性检测。结果表明 ,实验方法简便、快速 ,结果准确 ,可以用于 6 APA生产的质量控制。A method for the determination of main composition in degraded products of penicillin, 6 aminopenicillanic acid (6 APA),penicillin G kalium(PGK), phenylacetic acid(PAA), and the by product benzylpenicilloic acid(BPA) by HPLC was studied. Separation conditions were as follows: Spherisorb C\-\{18\} column, 250 mm×4 6 mm i d , 10 μm; mobile phase: V (methanol)∶ V (0 004 mol/L KH\-2PO\-4 buffer, pH 4 5)=50∶50; flow rate: 1 mL/min; detector: UV 230 nm. The method is effective, quick, accurate and reproducible. The satisfactory results show that this new method has certain practical values as an approach of quality control.

关 键 词：高效液相色谱法 6-氨基青霉烷酸 青霉素G钾 苯乙酸 青霉噻唑酸 青霉素酰化酶 青霉素裂解液 分析 

分 类 号：TQ465.1[化学工程—制药化工] TQ460.72[医药卫生—药物分析学]