血浆中有机磷农药的固相萃取-气相色谱法测定  

Determination of Organophosphate Pesticides in Human Plasma by Solid Extraction-Gas Chromatography

作　　者：冯翠玲[1] 周永新[1] 吴鹤松[1] 何耀忠[2] 

机构地区：[1]军事医学科学院毒物药物研究所,北京100850 [2]军事医学科学院307医院,北京100850

出　　处：《分析测试学报》2001年第3期78-80,共3页Journal of Instrumental Analysis

摘　　要：建立了固相萃取、GC -FPD(火焰光度检测器 )测定血浆中有机磷农药含量的方法。血样经固相萃取 ,GC -FPD分析 ,杂质干扰少 ,血中有机磷农药提取回收率在74 %以上 ,RSD在7.8 %～20 %。检出限 :敌敌畏、乐果为10μg/L ;甲拌磷、对硫磷为5μg/L。经实际中毒病人样品分析 ,证明该法操作简便、准确可靠 。A method for the determination of organophosphate pesticides, dichlorvos, phorate, dimethoate and parathion, in human plasma is described by solid extraction-gas chromatography with flame photometric detection. The recovery rates of all the pesticides from blood are above 74%, while the RSD is from 7.8% to 20.0%. The detection limit is 10 μg/L for phorate and parathion, and 5 μg/L for dichlorvos and dimethoate. There is little interference from impurities in the method. It has been used to detect samples from clinical poisoned patients, and proved to be simple, sensitive and accurate.

关 键 词：有机磷农药 固相萃取 诊断 气相色谱 血浆 农药中毒 临床检验 测定方法 

分 类 号：R595.4[医药卫生—内科学] R446.11[医药卫生—临床医学]