γ-干扰素诱导角质形成细胞凋亡及其Fas抗原表达研究  

Study on IFN-γ Induce Apoptosis of Keratinocytes and Express of Fas Antigen on the Keratinocytes

作　　者：张峻岭[1] 陈学荣[2] 殷金珠[3] 毛舒和[1] 

机构地区：[1]天津市长征医院皮肤科,天津300021 [2]北京医科大学第三临床医学院皮肤科 [3]北京医科大学免疫室

出　　处：《中国皮肤性病学杂志》2001年第3期163-164,166,共3页The Chinese Journal of Dermatovenereology

摘　　要：目的　探讨γ 干扰素在银屑病发病中的作用。方法　通过流式细胞仪测定角质形成细胞中亚二倍体细胞含量、片段化DNA分析及AnnexinV法检测经γ 干扰素诱导的角质形成细胞凋亡 ;采用组织化学、流式细胞仪测定经γ 干扰素作用后的角质形成细胞Fas抗原表达。结果　γ 干扰素上调角质形成细胞Fas抗原表达 (P <0 .0 1) ,诱导角质形成细胞凋亡 (P <0 .0 1)。结论　γ 干扰素可能通过上调角质形成细胞Fas抗原表达 ,进而诱导角质形成细胞凋亡而参与银屑病发病。Objective To investigate the role of IFN-γ in the pathogenesis of psoriasis.Methods Flow cytometric analysis quantification of hypodiploid cell,analysis of DNA fragmentation,Annexin V method,which study apoptosis of keratinocyte induced by IFN-γ.immunohischemistry and flow cytometry analysis the express of Fas antigen of keratinocyte induced by IFN-γ.Results IFN-γ upregulated the express of Fas antigen on the keratinocytes(P<0.01) and induce apoptosis of keratinocytes(P<0.01).Conclusion IFN-γ upregulated the express of Fas antigen on the keratinocytes and induce apoptosis of keratinocytes,which might be involved in the pathogenesis of psoriasis.

关 键 词：Γ-干扰素 角质形成细胞亡 FAS抗原 银屑病 

分 类 号：R758.630.2[医药卫生—皮肤病学与性病学]