冲击波和液体火箭推进剂中毒致冲毒复合伤大鼠实验模型的建立  

Establishment of the experimental rat model of combined injury caused by blast and liquid propellant intoxication

作　　者：岳茂兴[1] 杨鹤鸣[1] 李建忠[1] 徐世全[1] 杨志焕[2] 王正国[2] 夏亚东[3] 王德文[3] 彭瑞云[3] 

机构地区：[1]中国人民解放军第306医院 [2]重庆第三军医大学野战外科研究所 [3]中国人民解放军军事医学科学院

出　　处：《中华航空航天医学杂志》2001年第1期31-34,共4页Chinese Journal of Aerospace Medicine

基　　金：全军医学科学技术研究"十五"计划指令性项目! (2 0 0 0 0 2 8) ;总装备部卫生局重点指令性课题! (2 0 0 0 10 30 6 )

摘　　要：目的　复制冲击波和液体火箭推进剂中毒致冲毒复合伤的动物模型。　方法　健康雄性 Wistar大鼠 392只 ,随机分为 7组 :1冲击伤组 ;2偏二甲基肼 (U DMH)组 ;3四氧化二氮 (N2 O4)组 ;4冲击伤加 U DMH组 ;5冲击伤加 N2 O4组 ;6冲击伤加 U DMH及 N2 O4组 ;7对照组。每组动物又分为伤前、伤后 3、6、12、2 4、48、72 h7个时相组 ,每个小组 8只。　方法　冲击伤组用 BST- I型生物激波管致伤 ,冲击波超压为 471.5 k Pa,正压持续时间 5 5～ 6 0 m s。 UDMH及 N2 O4组分别吸入U DMH和 N2 O415 min,保持染毒柜中 U DMH的浓度为 80 0× 10 - 6 ,N2 O4的浓度为 43× 10 - 6 ,复合伤组动物则在冲击伤后 10 m in在染毒柜中吸入 UDMH和 (或 ) N2 O415 min,观察各组动物的临床状况并分别于 3、6、12、2 4、48、72 h时相活杀测定其生化指标和气血分析。　结果　动物观察和实验测定结果表明 ,上述方法复制的冲毒复合伤的动物模型具有与临床所见病人相近、稳定性、重复性好、经济实用等特点。　结论　利用生物激波管冲击和染毒柜染毒相结合的形式可以模拟复制现场液体火箭推进剂爆炸致冲毒复合伤的动物模型。这一模型的建立 。Objective To establish model of the blast-toxicosis combined injury caused by the explosion of the liquid propellant. Methods Three hundred and ninety-two male Wistar rats were divided randomly into 7 groups: ①blast injury group, ②unsymmetrical dimethylhydrazine(UDMH) group, ③nitrogen tetroxide(N 2O 4) intoxication group, ④blast injury accompanied with intoxication of UDMH group, ⑤blast injury accompanied with intoxication of N 2O 4 group, ⑥ blast injury accompanied with intoxication of UDMH and N 2O 4 group, ⑦normal controls. BST-I blast wave tube was used to induce blast injury, the excess pressure of the blast was 471.5 kPa and the positive pressure sustained for 55～60 ms. To establish the blast-toxicosis combined injury model, the UDMH 800×10 -6 (800 ppm) or/and the N 2O 4 43×10 -6 (43 ppm) were given to rats through inhalation in an intoxication tank for 15 min after the blast injury. The clinical states of experimental rats were observed, and the animals were killed sequentially 3, 6, 12, 24, 48 and 72 h after injury for blood gas analysis and biochemical examination. Results According to the clinical observation and results of blood gas analysis and biochemical examinations, the blast-toxicosis combined injury model was established successfully. It was stable, economical, practical and easy to repeat. Conclusions Injury caused by the explosion of liquid propellant can be simulated by using the blast wave tube and the intoxication tank. Establishment of the model is of great significance for the research work in this field.

关 键 词：爆震伤 气体中毒 大鼠 动物模型 

分 类 号：R858.3[医药卫生—航空、航天与航海医学]