用流式细胞仪检测血小板P-选择素和血小板-中性粒细胞复合物  

Detection of P-selectin and platelet-neutrophil complex in whole blood by flow cytometry

作　　者：张亚南[1] 王续武[1] 陆金红[1] 白丰沛[2] 

机构地区：[1]佳木斯大学第一临床医学院检验科 [2]佳木斯大学基础医学院

出　　处：《黑龙江医药科学》2001年第2期9-11,共3页Heilongjiang Medicine and Pharmacy

摘　　要：用流式细胞仪方法测定血小板的 ＣＤ６２Ｐ和 ＰＮＣ．选 ２５名健康不吸烟自愿者（男 １１名，女 １４名，年龄 ２２－６８岁，平均４２岁）．在用血小板激活物ＡＤＰ前后来评估流式细胞仪检测血小板的方法．ＣＤ６２Ｐ和ＰＮＣ在ＡＤＰ刺激后明显增高［分别为（８．０±２．７）％到（１８．８±６．４）％，Ｐ＜０．０１，（９．４±３．４）％到（１４．５±７．２）％Ｐ＜０．０１）］．论证，流式细胞仪测定血小板的方法是可行的，该方法简便，快速，敏感性高．Objective:To deter P-selectin (CD62P) and platelet-neutrophil complex (PNC) with flow cytometrer. Methods: Flow cytometric analysis was performed before and after platelet activation of adenosine diphosphate (ADP) in 25 healthy nonsmoking volunteers (11 males and 14 females, aging 22-68 years, mean 42 years). Results: The levels of CD62P and PNC were significantly increased after ADP activation [from (8.0 ± 2.7)% to (18.8 ± 6.4)%, P<0.01, from (9.4 ± 3.4)% to (14.5 ± 7.2)% P<0.01, respectively].Conclusion: The detection of CD62P and PNC with flow Cytometrer is feasible, specific, simple and accurate.

关 键 词：P-选择素 血小板-中性粒细胞复合物 流式细胞仪 ADP 

分 类 号：R446[医药卫生—诊断学]