大鼠闭合性脑损伤酯化银和白蛋白组织化学研究  

A study on pathological changes of closed diffuse brain injury in rats with H.E.,esterification－ silver stain and albumin immunohistochemical stain

作　　者：于晓军[1] 肖飞[2] 吴家馼[2] 吴梅筠[2] 

机构地区：[1]北华大学医学院法医学教研室,吉林吉林132001 [2]华西医科大学法医学院,四川成都610041

出　　处：《法医学杂志》2001年第2期69-73,76,共6页Journal of Forensic Medicine

基　　金：1994年度国家教委博士点基金!( No.C1037)

摘　　要：目的 研究闭合性脑损伤立即死亡和伤后 15min至 5天的大鼠脑组织病理变化。方法 应用 HE染色法观察闭合性脑损伤的组织形态学变化。结果 伤后立即死亡组大鼠脑干、大脑和小脑大量神经元表现为皱缩 (Ⅰ型变 )和肿胀 (Ⅱ型变 )，脑干大片神经纤维波浪样变形；伤后 2～ 8h，出现神经元水肿和轴索肿胀明显；伤后 8～ 24h，出现轴索收缩球。之后，Ⅰ型变、Ⅱ型变和收缩球随存活时间延长而增多加重。伤后 4～ 5天，组织水肿减轻或消失，收缩球仍存在。酯化银染色观察，各种损伤性改变均较 H.E.染色更清晰。 ABC法，血浆 Al随伤后时间延长，从血管周围逐渐向周围扩散，并进入损伤的神经元和胶质细胞。结论 脑震荡性损伤改变呈冲击性、对冲性和向心性分布。Objective To observed the pathological changes of closed diffuse brain injury in the rats died immediately and 15min to 5days after the injury. Methods H.E. staind and esterification－ silver stain were applied to investigate the closed diffuse brain injury.Results In rats died immediately after the concussive injury,a number of shrunken neurons(type I change),distended neurons(type II change)and wave－ like nerve fibers were identified in the brain tissue,especially in brain－ stem.At 2h and 8h after injury,brain edema and axonal swelling appeared clearly in the cortex and white matter,especially in brain－ stem.At post－ traumatic 8h and 24h,the axonal retraction balls began to appear.The amount of neurons undergoing type I and II changes and constraction balls increased along with the survivor time.After 4 days and 5 days,brain edema alleviated,but the retraction balls and axonal swelling still existed.With Esterifica－ tion silver stain,the above changes of neurons and nerve fibers were more obvious.With ABC stain,the distribution of albumin(Al) was extended from the perio－ vascular area to diffuse distribution.Al positive staining were more obvious in injuried neurons and nerve fibers.Conclusion The distribution of the concussive damage in the brain are coup,contra－ coup and centripental. [

关 键 词：闭合性脑损伤 病理改变 生物力学 法医学 

分 类 号：D919.4[医药卫生—法医学]