CODIS位点在排除亲权中的应用价值  

Study on application of CODIS loci to excluding paternity

作　　者：方建新[1] 刘锡杰 程大霖[1] 

机构地区：[1]司法部司法鉴定科学技术研究所,上海200063 [2]泉州市人民检察院,福建362000

出　　处：《法医学杂志》2001年第2期86-88,共3页Journal of Forensic Medicine

摘　　要：目的 对 CODIS位点 (FGA、 vWA、 CSF1PO、 TH01、 TPOX、 D3S1358、 D5S818、 D7S820、 D8S1179、 D13S317、 D16S539、 D18S51和 D21S11共 13个 STR位点 )在 100例排除亲权的亲子鉴定中的应用价值进行研究。方法 采用 Profiler Plus及 Cofiler荧光标记复合扩增系统，通过 310遗传分析仪对上述二个检测体系扩增产物的基因型进行分析。结果 在排除亲权的母亲－孩子－假设父亲三联体组中，所有观察案例其出现的排除指标数都在 3个以上，平均排除指标数为 6.63个；在假设父亲－孩子二联体组中， 94.0％的观察案例其排除指标数均在 3个以上，平均排除指标数为 5.01个。结论 CODIS位点在排除亲权的二联体和三联体组合亲子鉴定中，都符合鉴定应用要求 ;选择多态性较高的位点与增加排除指标存在直接的联系，以 DP、 H、 PE作为衡量 DNA位点应用价值的指标在具体鉴定实践中是可靠和可行的。Objective The study was carried out on application of CODIS loci (FGA, vWA, CSF1PO, TH01,TPOX, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51 and D21S11) in 100 cases of excluding paternity. Methods The PCR amplified products of Profiler Plus and Cofiler amplification kit were injected into a capillary on the ABI PRISM 310 Genetic Analyzer. GeneScan software analyzed the collected data, which can then be imported into Genotyper software for genotyping of alleles.Results In the group of Mother－ Child－ Alleged Father, more than 3 STR loci incompatibilities between alleged father and child were found in all observed cases, the mean incompatibility was 6.63 ; In the group of Alleged Father－ Child, 94.0％ of all cases observed was found more than 3 STR loci incompatibilities, the mean incompatibility was 5.01.Conclusion The results showed that CODIS loci had good application to excluding paternity, and the choice of hyperpolymorphic markers in investigation, which were valued by DP, H and PE, had direct relation with the augmentation of incompatibilities in excluding paternity. [

关 键 词：CODIS位点 短串联重复序列 亲子鉴定 排除亲权 法医鉴定学 

分 类 号：D919.4[医药卫生—法医学]