酵母激发子与水杨酸对Ti转化的丹参组培物内源激素含量的影响  

Effect of yeast elicitor and salicylic acid on contents of endogenous plant hormones in Ti-transformed Salvia miltiorrhiza cell aggregates

作　　者：李国婧[1] 王树才[1] 夏凯[1] 周燮[1] 

机构地区：[1]南京农业大学农学院,南京210095

出　　处：《南京农业大学学报》2001年第1期13-16,共4页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金资助项目 !(39870 473)

摘　　要：Ti转化的丹参组培物在MS NH4 培养基 (MS中不含硝酸铵 ,含蔗糖 30g·L-1)上经 2 5℃暗培养 18d ,生长量由大到小依次为 :对照 (CK) ,2 0 0 μmol·L-1水杨酸 (SA) ,4g·L-1酵母激发子 (E)和E +SA处理。其中CK和SA处理的组培物仅产生痕量的丹参酮类物质 ,而E和E +SA处理的总丹参酮产量分别增加到 12 2 3mg·L-1和 15 0 7mg·L-1,表明E处理能促进组培物产生丹参酮类物质 ,而SA可以强化E的这种作用。与上述差异有关的内源激素变化是 :培养 6～ 18d ,E +SA处理的ABA和iPAs含量均持续高于对照 ,分别增长了 2 8～ 9 8倍和 3 6～ 5 8倍 ,而GA1+ 3 和IAA含量分别比对照降低 13 2 %～ 5 6 9%和 34 8%～ 74 6 %。高水平的ABA和iPAs及低水平GA1+ 3 和IAA抑制了组培物的生长 。The fresh weight and dry weight of Ti transformed Salvia miltiorrhiza cell aggregates increase differently with various treatments,after being cultured in the MS NH 4 medium(MS without ammonium nitrate,containing 30 g·L -1 sucrose)under darkness for 18 days(25 ℃).The total tanshinones production reaches 12 23 mg·L -1 and 15 07 mg·L -1 by exogenous application of 4 g·L -1 of yeast elicitors(E)and E+200 μmol·L -1 of salicylic acid(SA)respectively.However,only trace amount of tanshinones was detected in the control and SA treatment,indicating that E could promote tanshinones production and SA enhances the effect of E.Compared with the control,the contents of ABA and iPAs in E+SA treated cell aggregates rise continuously and with an increase of 2 8?9 8 and 3 6?5 8 fold respectively,but those of GA 1+3 and IAA are decreased by 13 2%?56 9% and 34 8%?74 6% respectively,suggesting that high level of ABA and iPAs combined with low level of GA 1+3 and IAA inhibits the growth of cell aggregates,and therefore significantly stimulates the tanshinones production.

关 键 词：丹参 酵母激发子 水杨酸 丹参酮 内源植物激素 含量 Ti转化 药用植物 

分 类 号：S567.53[农业科学—中草药栽培]