植物afp表达载体的构建及其对农杆菌的直接转化  

Construction of plantafp expression vector and its directtransformation to agrobacterium

作　　者：尹明安[1] 崔鸿文[1] 樊代明[2] 郭立[3] 

机构地区：[1]西北农林科技大学园艺学院,陕西杨陵712100 [2]第四军医大学西京医院,西安710032 [3]西北农林科技大学生命科学学院,陕西杨陵712100

出　　处：《西北农业大学学报》2001年第2期1-5,共5页Journal of Northwest Sci-Tech University of Agriculture and Forestry（Natural Science Edition)

基　　金：陕西省科技攻关项目! (98K12 -G19(11) )

摘　　要：详细报道了胡萝卜抗冻蛋白基因 (afp)植物表达载体的构建过程及其直接法转化农杆菌的方法。以限制性内切酶从克隆载体上切取 afp基因 ,将其连接在相应酶切的 p BI12 1工程质粒上 ,构建成 afp植物表达载体。采用直接转化法将重组子导入根癌农杆菌 ,并用Construction of a vector expressing carrot antifreeze protein gene(afp) in plants and its direct transformation to agrobacterium were thoroughly reported.Clone vector was digested with restriction endonucleases,and the acquired afp was ligated to engineered plasmid p BI1 2 1 which was digested with appropriate restriction endonucleases,and therefore a plant expression vector for carrot afp was constructed.The recombinant was transferred into agrobacterium tumefacien by direct DNA transfer and the transformant was identified with PCR.

关 键 词：植物抗冻蛋白 农杆菌 胡萝卜 遗传转化 表达载体 构建过程 转化方法 

分 类 号：S188[农业科学—农业基础科学] Q785[生物学—分子生物学]