鹅观草属、披碱草属、猬草属和仲彬草属植物的RAPD分析及其系统学意义  

Phylogenetic Relationships Among Species of Roegneria, Elymus, Hystrix and Kengyilia Based on Random Amplified Polymorphic DNA

作　　者：周永红[1] 郑有良[1] 杨俊良[1] 颜济[1] 魏育明[1] 杨瑞武[1] 

机构地区：[1]四川农业大学小麦研究所,四川都江堰611830

出　　处：《四川农业大学学报》2001年第1期14-20,共7页Journal of Sichuan Agricultural University

基　　金：四川省教育厅自然科学基金项目 ;四川省科技厅科研项目

摘　　要：利用随机扩增多态性DNA(RAPD)技术分析了鹅观草属 ( 7种 )、披碱草属 ( 3种 )、猬草属 ( 2种 )和仲彬草属 ( 3种 ) 4个属的 15种植物。对 34个OPRON公司十聚体随机引物进行多态性筛选 ,2 7个 ( 79 4% )能产生多态性。 16个引物产生的 2 13个DNA片断 ,用于计算种间Nei氏遗传相似性系数分析 ,在NTSYS程序中利用UPGMA构建系统发育树状图。分析结果表明 :①这 4个属的物种各自聚类在一起 ,在相似系数 0 6水平上 ,明显分为 4组 ,分别代表了Hystrix、Elymus、Roegneria和Kengyilia 4个属 ;②Hystrix的 2个物种与Roegneria和Kengyilia物种的亲缘关系较远 ,与同具StH基因组的Elymus也有较大的RAPD变异 ;③Roegneria与Elymus亲缘关系较近 ;④Roegneria中StY和StYH基因组组合的物种亲缘关系很近 ,StY基因组的物种间存在着RAPD遗传变异 ;⑤Kengyilia与Roegneria亲缘关系较近 ;⑥RAPD结果与形态学和细胞学等分析结果一致 ,支持将它们独立作为属分类等级来处理。Fifteen species of Roegneria, Elymus, Hystrix and Kengyilia (Poaceae: Triticeae) were analyzed by using random amplified polymorphic DNA (RAPD) markers. 34 decamer oligonucleotides random primers from Opron Technologies were used for polymorphic selection. 27 (79 4%) produced polymorphic products. A total of 213 bands amplified from 16 primers were selected for RAPD analysis. The data were used to generate Nei's similarity coefficients and to construct a dendrogram by using UPGMA in NTSYS programs. It is concluded as follows: ①The species of Roegneria, Elymus, Hystrix and Kengyilia are clustered in four groups, which represent Roegneria, Elymus, Hystrix and Kengyilia respectively on 0 6 of genetic similarity coefficients; ②The relationships between the species of Hystrix and the species of Roegneria and Kengyilia are remote; ③Genetic diversity exists between Elymus and Hystrx although they possess similar StH genome; ④The species of StY and StYH genomes in Roegneria are clustered together, which suggests they have closer relationships; ⑤There is certain RAPD genetic diversity in the species of StY genomes of Roegneria; ⑥Kengyilia is related to Roegneria; ⑦RAPD results are basically comparable with those obtained from studies on morphology and cytology. The present study discusss the usefulness of RAPD markers in the systematic studies of Triticeae.

关 键 词：鹅观草属 披碱草属 仲彬草属 猬草属 RAPD分析 亲缘关系 基因组 系统学 

分 类 号：Q949.714.2[生物学—植物学] Q943