氯苯类化合物的生物降解  

Experiment of Biodegradation of Chlorobenzenes

作　　者：甘平[1] 樊耀波[1] 王敏健[1] 

机构地区：[1]中国科学院生态环境研究中心,北京100085

出　　处：《环境科学》2001年第3期93-96,共4页Environmental Science

基　　金：国家自然科学基金!资助项目 (2 96 770 2 7) ;环境模拟与污染控制国家重点联合实验室开放基金

摘　　要：经过 2个月的驯化 ,从某染料厂和某毛纺厂活性污泥中分离出能够生长于 1 ,4 二氯苯、1 ,2 ,4 三氯苯和六氯苯的 4种微生物 .通过测定该混合菌降解氯苯类化合物过程中的累积好氧量、微生物生长曲线及降解产物Cl-的释放 ,证明在好氧条件下该混合菌能够以 1 ,4 二氯苯和 1 ,2 ,4 三氯苯为唯一碳源和能源 ,降解产物Cl-浓度的变化与微生物生长周期有关 .通过好氧振荡瓶培养法测得 3种氯苯的生物降解顺序为 :1 ,4 二氯苯 [356 7μg (L·d) ]>1 ,2 ,4 三氯苯 [1 1 0 .4 μg (L·d) ]>六氯苯 [～ 6μg (L·d) ],说明氯取代数越多 ,氯苯类化合物越难被好氧降解 .Four strains of microorganisms which were able to grow at the presence of 1,4-dichlorobenzene 1,2,4-trichlorobenzene and hexachlorobenzene were isolated from the sludge collected from two different sites after incubating about 2 months. During the growth of the mixed microorganisims on chlorobenzenes, the accumulating consumption of oxygen, the microbial population curve and the concentration of released Cl - were investigated. The result showed that the mixed microorganisms were able to utilize 1,4-dichlorobenzen and 1,2,4-trichlorobenzene as sole carbon source and energy source. The concentration of released Cl - may depend strongly on the activity of the living cells. The order in which the chlorobenzenes were readily degraded was 1,4-dichlorobenzenez[356.7μg/(L·d)]>1,2,4-trichlorobenzene[110.4μg/(L·d)]>hexachlorobenzene[～6μg/(L·d)].The number of the substituted chlorine on the chlorobenzenes directly resulted in the resistance to biodegradation.

关 键 词：氯苯类化合物 二氯苯 三氯苯 六氯苯 生物降解 

分 类 号：X783[环境科学与工程—环境工程]