α-萘酚对小球藻谷胱甘肽及其还原酶的影响  

Effects of --naphthol on the glutathione and its reductase in Chlorella

作　　者：彭金良[1] 严国安[1] 沈国兴[1] 邓晓军 

机构地区：[1]武汉大学环境科学与工程系,湖北武汉430072

出　　处：《中国环境科学》2001年第2期140-143,共4页China Environmental Science

基　　金：国家自然科学基金资助项目!(39870152)

摘　　要：研究了--萘酚对2种小球藻谷胱甘肽-包括还型谷胱甘肽GSH与氧化型谷胱甘肽GSSG-含量及其还原酶-GR-活性的影响.结果表明,低浓度--萘酚对藻细胞的谷胱甘肽水平和GR活性有显著的激发作用,随着--萘酚浓度的升高,普通小球藻和蛋白核小球藻的GSH含量、谷胱甘肽总量及GR活性均有所提高,并分别在5mg/L和10mg/L时达到最大值,而GSSG均不断下降且在相同浓度下-2 mg/L-至最小后开始上升.GSSG/GSH先降后升的变化说明藻细胞在--萘酚胁迫下膜脂过氧化加剧,而GSH、GR在清除活性氧、消除过氧化方面起了重要作用.：The effects of --naphthol on the contents of GSH and GSSG and the activity of GR -glutathione reductase -in Chlorella vulgaris and Chlorella pyrenoidosa were studied. The results showed that the levels of GSH and GR activities were induced markedly by --naphthol at low concentrations. The contents of GSH, total amount of glutathione and the activity of GR increased with the enhancement of concentrations of --naphthol, and reached their maximum values at 5mg/L and 10mg/L respectively, but the contents of GSSG decreased and reached their minimum values at same concentration -2mg/L- and increased afterward. The change of GSSG/GSH showed that the lipid peroxidation aggregated. GSH and GR played important role in clearing the active oxygen and prevent lipid peroxidation .

关 键 词：Α-萘酚 普通小球藻 蛋白核小球藻 谷胱甘肽 谷胱甘肽还原酶 环境污染物 

分 类 号：X503.2[环境科学与工程—环境工程] X592