假单胞菌JX165及其完整细胞对硝基苯的好氧降解  

Aerobic degradation of nitrobenzene by Pseudomonas sp. JX165 and its intact cells

作　　者：王竞[1] 周集体[1] 张劲松[1] 张爱丽[1] 吕红[1] 

机构地区：[1]大连理工大学环境科学与工程学院,辽宁大连116012

出　　处：《中国环境科学》2001年第2期144-147,共4页China Environmental Science

基　　金：教育部跨世纪优秀人才培养基金资助项目!(教技函1999-2)

摘　　要：利用Pseudomonas sp. JX165及其完整细胞考察了硝基苯的好氧降解特性,并对其降解机理进行了分析.结果表明,菌株JX165对硝基苯的好氧降解过程,首先还原产生2-氨基酚和苯胺,再经不同的氧化途径彻底降解;在高浓度-＞200mg/L-及高转速-＞200r/min-下,菌株JX165对硝基苯的降解过程会产生更难以降解的偶氮苯类化合物;经硝基苯诱导的JX165细胞中含有硝基苯、2-氨基酚、苯胺及邻苯二酚降解酶,且它们均为诱导酶,其动力学参数分别为Km-硝基苯-=34.12mg/L,Vmax-硝基苯-=3.01mg/-L-min-;Km-2-氨基酚-=43.49mg/L,Vmax-2-氨基酚- =2.56mg/-L-min-;Km-邻苯二酚-=51.03mg/L,Vmax-邻苯二酚-=1.32mg/-L-min-;Km-苯胺-=57.15mg/L,Vmax-苯胺-=0.79mg/-L-min-.The aerobic degradation characteristics and mechanism of nitrobenzene by Pseudomonas sp.JX165 and its intact cells were investigated and analyzed. The results indicated that, during the course of degradation, 2-aminophenol and aniline were first produced by reduction, then the degradation was completed by different oxidation pathway; at high concentration (more than 200 mg/L) and high shaking speed (more than 200 r/min), a more refractory azobenzene would be produced;the JX165 cells induced by nitrobenzene contain degrading enzymes for nitrobenzene, aniline,2-aminophenol and catechol, and all were inducible, with kinetic parameters: Km(nitrobenzene)=34.12mg/L, Vmax(nitrobenzene)=3.01mg/L·min; Km(2-aminophenole)=43.49mg/L, Vmax(2-aminophenol)=2.56 mg/(L·min); Km(catechol)=51.03mg/L, Vmax(catechol)=1.32 mg/(L·min); and Km(aniline)=57.15mg/L, Vmax(aniline)=0.79mg/(L·min) respectively.

关 键 词：假单胞菌 硝基苯 好氧 降解 降解机理 动力学 有机废物处理 

分 类 号：X783[环境科学与工程—环境工程] X172