RT-PCR检测猪传染性胃肠炎病毒  

Detection of Transmissible Gastroenteritis Virus (TGEV) by Reverse Transcription-Polymerase Chain Reaction

作　　者：李军[1] 林继煌[1] 陆承平[2] 何孔旺[1] 倪艳秀[1] 还红华[1] 钱永清[3] 

机构地区：[1]江苏省农业科学院农业部畜禽疫病诊断重点开放实验室,江苏南京210014 [2]南京农业大学动物医学院,江苏南京210095 [3]上海农业科学院畜牧兽医研究所,上海201106

出　　处：《中国兽医学报》2001年第3期246-248,共3页Chinese Journal of Veterinary Science

摘　　要：根据猪传染性胃肠炎病毒 ( TGEV)纤突蛋白 S基因的 5′端核酸序列设计了 1对引物。该对引物扩增的目的片段长度为 886bp。在优化 RT-PCR反应条件的基础上 ,建立了检测 TGEV的 RT-PCR方法。用此RT-PCR方法检测 56份猪腹泻粪样 ,同时用夹心 ELISA法作对照。结果显示 ,RT-PCR法检测的 2 0份阳性粪样 ,用夹心 ELISA法检测有 1 4份呈阳性 ,两者的符合率为 89%。 RT-PCR法比夹心 ELISA法敏感性更高 ,可用于A pair of oligonucleotide primers appropriate for PCR amplication were selected based on the two conserved regions of the 5′ end of the gene encoding the S protein of TGEV.A reverse transcription polymerase chain reaction(RT PCR) assay was developed for the detection of TGEV after optimization of the reaction conditions.The sandwich ELISA was also used for control.Out of 56 fecal samples,20 were positive detected by RT PCR,meanwhile only 14 were positive detected by sandwich ELISA.The corresponding rate of the two methods was 89%.It suggests that RT PCR is more sensitive than sandwich ELISA and should be quite useful for detection of TGEV and the epidemiological survey of TGE.

关 键 词：传染性胃肠炎病毒 RT-PCR 猪 诊断方法 

分 类 号：S858.28[农业科学—临床兽医学] S852.65[农业科学—兽医学]