欧鳗“狂游症”病毒的细胞定位与超微病变  

Cellular distribution and pathological ultramicro-structural change of “mad swimming symptoms” virus of Anguilla anguilla

作　　者：颜江华[1] 颜青[1] 黄印尧[2] 倪子绵[2] 陈信忠[2] 方莹[1] 

机构地区：[1]厦门大学生命科学学院抗癌研究中心,福建厦门361005 [2]厦门出入境检验检疫局,福建厦门361012

出　　处：《台湾海峡》2001年第1期54-56,T001,共4页Journal of Oceanography In Taiwan Strait

基　　金：国家自然科学基金资助项目 !(3980 0 10 7);福建省自然科学基金资助项目!(C9810 0 4)

摘　　要：应用电镜标本制备和观察技术对 45尾“狂游症”欧鳗的主要脏器进行系统观察 ,在脑、心脏、肌肉和皮肤组织中 ,发现一种致病性病毒 ,多数病毒定位于细胞的内质网腔 ,一般为球状或椭圆球状 ,大小约为 5 0～ 80nm× 1 0 0～ 1 5 0nm ,具有囊膜 ,囊膜外附有纤突 ,可以造成脑细胞的肿胀、空泡变性和心肌细胞线粒体的结构破坏和空泡化等细胞病变 .人工感染欧鳗中找到同样病毒 ,表明该病毒是欧鳗“狂游症”With techniques of sample preparation and observation of the transmission electron microscope, main organs and tissues of 45 Anguilla anguilla suffered from “mad swimming symptom” were observed and analyzed systemically. A pathogen virus had been found in the cells of brain, heart, muscle, and skin. Most of virions are located at cavity of endoplasmic reticulum with common shapes of sphericity or ellipse. Its size is about 50～80nm×100～150nm with an envelope to which peplomeres are adherent. The pathologic changes of the swelling and vesiculating in brain cells and structure destroying and vesiculating in heart cells were observed in suffering eels. The same virions had been found in the eels infected artificially. It showed that the virus is the pathogen of Anguilla anguilla with “mad swimming symptom”.

关 键 词：欧鳗 狂游症病毒 电镜标本 细胞定位 超微病变 

分 类 号：S941.41[农业科学—水产养殖]