不同虾种间肌肉组织蛋白质的比较  

Comparative study on museular protein in six species of shrimp

作　　者：许玉德[1] 孙晟[2] 朱云[1] 

机构地区：[1]厦门大学生命科学学院,福建厦门361005 [2]中国科学院海洋研究所,山东青岛266071

出　　处：《台湾海峡》2001年第2期177-181,共5页Journal of Oceanography In Taiwan Strait

摘　　要：采用薄层等电聚焦凝胶电泳方法对罗氏沼虾 (Macrobrachiumrosenbergii)、绿须虾 (Aristeusvirilis)、长毛对虾 (Penaeuspenicillatus)、日本对虾 (P .japonicus)、斑节对虾 (P .monodon)和周氏新对虾 (Metapenaeusjoyneri)的肌肉组织蛋白质进行分离 ,检测各蛋白质组份及其等电点 .根据蛋白质区带的共享度计算其相互间的遗传距离指数 ,然后用聚类方法处理数据 ,构建系统树 ,分析其间的差异程度 ,最后确定 6种虾的亲缘关系 .结果表明 ,每种虾可获得 6～ 1 1条蛋白质区带 ,等电点范围在 4～6 .95之间 .虾类肌肉组织蛋白质组份及其电泳行为 ,可为虾类在分类学上的定位提供生化依据 .Museular protein of six species shrimp ( Macrobrachium rosenbergii, Aristeus virilis, Penaeus penicillatus, P. japonicus, P. monodom and Metapenaeus joyneri ) were detected using polyacrylamide gel electrophoresis method. The results showed that there were 6～11 electrophoresis bands of protein on these shrimp. Index of genetic similarity( F ) was calculated. The value of (1- F ) was used to evaluate genetic distances between species and to construct phylogenetic tree. These protein analysis was consistent with extant taxonomic system of shrimp group. Therefore, overall results revealed phylogenetic relationship of differential taxonomic class of shrimp group on protein.

关 键 词：蛋白质 等电聚焦电泳 绿须虾 长毛对虾 日本对虾 斑节对虾 肌肉组织 蛋白质 罗氏沼虾 

分 类 号：Q959.223.5[生物学—动物学]