血液样品中微量砷的简便石墨炉原子吸收测定法  

Determination of Arsenic in Blood by an Electrothermal Atomic Absorption Spectrophotometer

作　　者：李友[1] 刘宝军[1] 何公理[1] 吉荣娣[1] 

机构地区：[1]中国预防医学科学院环境卫生与卫生工程研究所,北京100050

出　　处：《现代科学仪器》2001年第2期49-52,共4页Modern Scientific Instruments

基　　金：国家自然科学基金资助! (No 39660 0 71 )

摘　　要：在酸浸出法测定镉含量的基础上 ,用超声处理血液样品 ,建立了一种简便的石墨炉原子吸收分光光度法来测定血液样品中的微量砷。通过不同基体改进剂的比较 ,确定了镍作为测定砷的基体改进剂 ,1 0mL血液样品用 0 1mL 8mol/LHCl浸泡样品 ,超声处理 30分钟后测定 ,其检出限为 3 0 3ng/mL ;线性范围为 5 0～ 5 0 0ng/mL ;精密度为 2 5 % ;回收率在 88 0 %～ 98 1%之间。研究表明该法测定血液中的微量砷 ,具有简单、灵敏、精密、准确等特点。用本法测定了慢性砷中毒小鼠红细胞中的砷含量 。A simple method of determining arsenic in blood by electrothermal atomic absorption spectrophotometer was developed based on the modification of the method of extraction with acid. A Ni(NO 3) 2 matrix modifier was used to thermally stabilize arsenic. After comparing to the wet digestion method, the result suggests that this method is relatively simple, accurate, precise and sensitive. The detection limit is 3 03 ng/mL, the precision is 2 5% and the recovery in the range of 88 0%～98 1%. The concentration of arsenic in blood was determined by this method. The results show that the concentration of arsenic in blood of the arsenism mice is higher than that in the control mice.

关 键 词：砷 血液 石墨炉原子吸收法 测定 

分 类 号：O657.3[理学—分析化学]