抗苯并咪唑类杀菌剂的香蕉潜伏炭疽菌β-微管蛋白DNA测序  

Cloning and Sequencing of β-Tubulin Gene of Thiabendazole Resistant Strains of Colletotrichum musae in Banana Fruit

作　　者：杨叶[1] 黄圣明[1] 

机构地区：[1]华南热带农业大学植物保护学院,海南儋州571737

出　　处：《热带作物学报》2001年第1期42-47,共6页Chinese Journal of Tropical Crops

基　　金：华南热带农业大学基金

摘　　要：将经特克多诱导后获得抗性的２个Ｃｏｌｌｅｔｏｔｒｉｃｈｕｍ　ｍｕｓａｅ　菌系，及其相应的未经任何药剂处理的敏感菌系的ＤＮＡ作为模板，用真菌β－微管蛋白基因的２个引物进行ＰＣＲ扩增，均得到一段长８７４ ｂｐ的β－微管蛋白的编码序列，并含有 １个５３ ｂｐ的内含子。通过ＰＣ／Ｇｅｎｅ软件分析，与已报道的其它５种真菌β－微管蛋白基因相比，核苷酸同源性最高达９７．８％，翻译成氨基酸同源性最高达 ９９．３％；抗性菌系与敏感菌系相比，在 １９８和 ２００位位置的氨基酸并没有发生突变，但在１８６、２３２、３１３和３２９位置氨基酸发生了变异。The total DNA isolated from TBZ-resistant and sensitive isolates of C.musae was used as templates in PCR amplification. The PCR product was cloned into T-plasmid and then sequenced. Homology comparison using PC/Gene software showed that the sequence of this fragment had a high homology to the sequences of β -tubulin genes of other 5 fungi reported earlier. TBZ- resistant isolates did not carry the allelic mutation at amino acid codes 198 and 200 of β- tubu-lin gene when compared to the sensitive isolates. But the amino acid changed in codens 186, 232, 313 and 328 of this fragment.

关 键 词：抗药性 β-微管蛋白基因 序列分析 炭疽病菌 苯并咪唑 杀菌剂 香蕉 

分 类 号：S436.68[农业科学—农业昆虫与害虫防治] S432.42[农业科学—植物保护]