苯并(a)芘对大弹涂鱼肝脏芳烃羟化酶活性的影响  

Effects of benzo(a)pyrene exposure on aryl hydrocarbon hydroxylase activities in the liver of Boleophthalmus pectinirostris

作　　者：冯涛[1] 郑微云[1] 洪万树[2] 张其永[2] 

机构地区：[1]厦门大学环境科学研究中心,福建厦门361005 [2]厦门大学海洋系,福建厦门361005

出　　处：《水产学报》2001年第2期156-160,共5页Journal of Fisheries of China

基　　金：国家自然科学基金资助项目 ( 4 9876 0 2 9)

摘　　要：研究了苯并 (a)芘 (BaP)胁迫下大弹涂鱼肝脏芳烃羟化酶 (AHH)活性的变化 ,结果显示 :BaP对大弹涂鱼肝脏AHH活性总体表现为显著的诱导作用 (P <0 .0 1) ,其中BaP暴露的浓度及时间是影响AHH活性的重要因素。 0 .0 5mg·L-1浓度的BaP暴露对AHH活性无显著影响 ,而 0 .2mg·L-1和 0 .5mg·L-1浓度组AHH活性则显著被诱导 (P <0 .0 1)。随着暴污时间的延长 ,0 .2mg·L-1浓度组AHH活性被显著诱导 (P <0 .0 1) ,而 0 .5mg·L-1组AHH活性则相对稳定。对AHH活性变化的剂量 效应关系进行回归分析的结果表明 ,0 .5mg·L-1浓度组暴露 7d时 ,AHH活性受到一定程度的抑制。污染解除后 ,0 .5mg·L-1浓度组AHH活性显著降低 ,恢复至与对照组相近 ,表明大弹涂鱼肝脏仍具有较强的生理调节机能 ,同时也表明AHH活性可及时反映环境中BaP的水平。以上这些结果表明AHH适于作为大弹涂鱼受BaP胁迫的生物指标。Changes of aryl hydrocarbon hydroxylase(AHH) activities in the liver of Boleophthalmus pectinirostris with benzo(a)pyrene(BaP) exposure were detected in experimental condition. The results showed that the activities of AHH were significantly induced by BaP(P<0.01). Factors including BaP concentration and exposure time had important effect on AHH activities. The activities of AHH decreased to the control level after BaP was removed. It turned out that the physiological modulatary mechanism was still exist in the liver of B. pectinirostris and the activities of AHH could reflect the level of BaP in the environment. All of the results showed that AHH was suitable to be the bioindicator of BaP exposure.

关 键 词：苯并(A)芘 大弹涂鱼 芳烃羟化酶 肝脏 海洋污染 生物指标 

分 类 号：X835[环境科学与工程—环境工程] X826