人趋化因子样因子-1的体内表达对BXSB狼疮鼠发病影响的研究  

Effect of human chemokine-like factor-1 expression in vivo on the development of systemic lupus erythematosus in BXSB mice

作　　者：徐志伟[1] 邓鸿业[2] 马大龙[2] 

机构地区：[1]潍坊医学院免疫学研究所,261042 [2]北京大学医学部免疫学系

出　　处：《中华风湿病学杂志》2001年第3期162-164,共3页Chinese Journal of Rheumatology

摘　　要：目的　初步探讨人趋化因子样因子 1(humanchemokine likefactor 1,hCKLF 1)在系统性红斑狼疮BXSB小鼠发病中的作用。方法　利用肌肉介导的电脉冲转基因技术 ,便重组的真核表达质粒pCDI hCKLF 1在BXSB小鼠体内表达 ,并检测血清中IgM和IgG型抗DNA抗体的水平 ,尿蛋白浓度和血清尿素氮 (BUN)浓度。结果　hCKLF 1的体内表达对雌雄BXSB小鼠IgM和IgG型抗DNA抗体的产生无明显影响 ,但在短时间内显著地促进雄性BXSB小鼠尿蛋白浓度的异常升高 ,加重其肾脏炎症。结论　hCKLF 1可能是参与狼疮肾炎的介质之一 。Objective To probe into roles of hCKLF 1 in the development of systemic lupus erythematosus in BXSB mice in vivo .Methods The recombinant eukaryotic expression plasmid for hCKLF 1 (pCDI hCKLF 1) was transferred and expressed in lupus prone BXSB mice with the technique of muscle mediated transgene by electric pulse;the levels of serum IgM and IgG anti DNA antibodies as well as serum BUN and urine protein were detected.Results hCKLF 1 expression in vivo did not make any effects on the levels of IgM and IgG anti DNA antibodies in female and male BXSB mice,but markedly enabled abnormal elevation of urine protein in male BXSB mice in short time,suggesting its ability for accelerating glomerulonephritis.Conclusion hCKLF 1 may be one of inflammation mediators,playing an important role in the lupus glomerulonephritis of male BXSB mice,and its target cells may be monocyte and macrophage.

关 键 词：系统性红斑狼疮 趋化因子 狼疮肾炎 小鼠 

分 类 号：R593.241[医药卫生—内科学]