白介素-10基因多态性与术后脓毒症发生发展的相关研究  

Genetic polymorphism within the interleukin-10 gene promoter in patients with severe sepsis

作　　者：方向明[1] 舒强[2] 唐明山[2] 陈齐兴[1] Frank Stueber 

机构地区：[1]浙江大学医学院附属邵逸夫医院麻醉科,浙江杭州310016 [2]浙江大学医学院附属儿童医院 [3]德国波恩大学麻醉与危重病医学科

出　　处：《中国危重病急救医学》2001年第5期265-268,共4页Chinese Critical Care Medicine

基　　金：国家自然科学基金资助项目! (No.3 0 0 0 0 15 5 );教育部留学回国人员科研启动基金资助项目!(No.1999747)

摘　　要：目的 :研究白介素 10 (IL 10 )基因启动子区域中 IL 10 5 92、IL 10 819、IL 10 10 82基因多态性与术后严重脓毒症易感性及其预后的相关性。方法 :采用聚合酶链反应 (PCR)结合 Rsa 、Mae 、Mnl 限制性内切酶酶切分析法检测 116例术后并发严重脓毒症患者和 141例健康献血员 (对照组 )的 IL 10 5 92、IL 10 819、IL 10 10 82基因多态性。结果 :IL 10 10 82等位基因 1的频率在脓毒症组为 5 9.9% ,对照组为 5 0 .4% (P<0 .0 5 ) ;IL 10 10 82基因型分布频率在脓毒症组和对照组间有显著性差异 (P<0 .0 5 ) ;而IL 10 5 92、IL 10 819等位基因频率及基因型频率在脓毒症组和对照组间均无显著性差异 (P均 >0 .0 5 ) ;IL 10 5 92、IL 10 819、IL 10 10 82等位基因频率及基因型频率在死亡的与存活的脓毒症患者间均无显著性差异 (P均 >0 .0 5 )。结论 :IL 10 10 82基因多态性与术后严重脓毒症的易感性有关 ,与其预后不相关 ;而IL 10 5 92、IL 10 819基因多态性与术后严重脓毒症的易感性及预后均不相关。Objective:To investigate whether three biallelic polymorphisms at the position 592,819 and1082 in the promoter region of the IL10 gene were associated with the incidence and outcome of severe sepsis.Methods:The IL10 592,819 and 1082 polymorphisms were typed by polymerase chain reaction followed by digestion with the RsaⅠ,MaeⅢ and MnlⅠ restriction enzyme respectively.Results:Patients with severe sepsis had a significant elevation of IL10 1082 allele 1 compared with that in controls (59.9% vs.50.4%,P<0.05).Genotype distribution of the IL101082 polymorphism significantly differed between patients and controls (P<0.05).However,the allele frequencies and genotype distribution of the IL10 1082 polymorphism did not differ between surviving and nonsurviving patients (P>0.05).No significant differences in the genotype distribution and allele frequencies of the IL10 592 and IL10 819 polymorphisms were observed between patients with severe sepsis and healthy controls,and between surviving and nonsurviving patients (P>0.05).Conclusions:The polymorphism at position 1082 in the promoter region of the IL10 gene may be associated with the susceptibility to severe sepsis.In contrast,the additional two high linked IL10 polymorphisms are neither associated with the incidence nor with the outcome of severe sepsis.

关 键 词：脓毒症 白介素-10 基因多态性 

分 类 号：R631.2[医药卫生—外科学]