外源性表皮生长因子促进鼠坐骨神经再生的实验研究  

Promotion of nerve regeneration in rat sciatic nerve by exogenous epidermal growth factor: an experimental study

作　　者：张烽[1] 顾玉东[1] 徐建光[1] 李继峰[1] 

机构地区：[1]上海复旦大学华山医院手外科,现在南通医学院附属医院骨科200040

出　　处：《中华手外科杂志》2001年第2期105-107,共3页Chinese Journal of Hand Surgery

基　　金：上海市领先学科基金资助项目!(95 Ⅲ 1 ) ;卫生部重点学科基金资助项目!(96 3);美国中华医学基金资助项目!(95 61 9);江苏省青

摘　　要：目的　评价外源性表皮生长因子 (exogenousepidermalgrowthfactor ,EGF)对神经再生的影响。方法　雄性SD大鼠 48只 ,建立成鼠坐骨神经挤压伤模型。按术后注射药物的不同分成 2组 ,每组 2 4只鼠。损伤对照组 :在神经损伤处注射生理盐水 5 μl ;EGF组 :注射EGF/生理盐水液 ( 10 μg /5 μl)。于术后2、4、6周 3个时间点测定坐骨神经功能指数、CMAP的潜伏期、最大诱发电位的恢复率、组织学检测、电镜超微结构观察。结果　坐骨神经功能指数恢复率在各时间点 ,EGF组明显优于对照组 (P <0 .0 1) ;CMAP潜伏期的延迟率 ,EGF组明显小于对照组 (P <0 .0 1) ;诱发电位恢复率EGF组明显好于对照组(P <0 .0 1)。组织学检查 :有髓神经纤维数在术后 2、4周时EGF组明显多于对照组 (P <0 .0 1) ;各时间点有髓神经纤维直径及截面积 ,EGF组明显优于对照组 (P <0 .0 1)。超微结构观察 :EGF组再生神经的有髓纤维数 ,髓鞘厚度 ,髓鞘的成熟度明显好于对照组。结论　外源性EGF对神经的再生和功能恢复有一定的作用。Objective To evaluate the effect of epidermal growth factor (EGF) on promoting nerve regeneration. Methods Sciatic nerve crush injury model was set up in 48 female Sprague Dawley rats. They were randomly divided into two groups according to different medication, with 24 each. Injection of normal saline (5ul) and EGF/normal saline (10 μg/5 μl) was done at the site of nerve injury in the control group and EGF group respectively. Measurement of sciatic functional index (SFI), latency of CMAP, and recovery rate of the maximal evoked potential, and histomorphometric and ultra structural observation were carried out 2, 4, and 6 weeks after the operation respectively. Results Compared with the control group, there was significant improvement in sciatic functional index, latency of CMAP, and recovery rate of evoked potential at different assessment intervals. The number of regenerated myelinated nerve fibers in 2 and 4 weeks of postoperative intervals in EGF group was much more than that in control group. The mean diameter and cross sectional area of regenerated myelinated nerve fiber was larger in the EGF group than in the control group in all time intervals. Myelin sheath thickness and maturity was superior in EFG group under electron microscopic observation. Conclusions Exogenous epidermal growth factor (EGF) can play a certain role in nerve regeneration and restoration of nerve function.

关 键 词：神经再生 生长物质 坐骨神经 表皮生长因子 

分 类 号：R651.3[医药卫生—外科学]