混浊介质荧光显微成像的层析能力研究  

Study on Laminating Ability of Fluorescent Microscopic Imaging for Turbid Media

作　　者：鲁强[1] 曾绍群[1] 骆清铭[1] 阮玉[1] 

机构地区：[1]华中理工大学生物医学光子学研究所,湖北武汉430074

出　　处：《光电工程》2001年第3期16-20,共5页Opto-Electronic Engineering

基　　金：国家自然科学基金资助项目 (3 9870 2 0 5 )

摘　　要：采用蒙特卡罗方法给出了混浊介质显微成像中 ,共焦荧光、双光子激发荧光的空间分布。该分布表明双光子激发荧光显微成像具有内在的轴向层析能力 ,而共焦荧光成像的轴向层析能力依赖于共焦针孔的采用。The spatial distribution of the confocal fluorescence and the two photon excitation fluorescence in turbid media are studied with Monte Carlo simulation.These distributions show that the microscopic imaging of the two photon excitation fluorescence has an inherent axial laminating ability but the axial laminating ability for the confocal fluorescence imaging relies on the application of confocal pinhole.The further investigation demonstrates that the confocal pinhole also has the property for improving laminating ability of the two photon excitation microscopic imaging.

关 键 词：荧光显微术成像 混浊介质 层析能力 

分 类 号：TH742.65[机械工程—光学工程]