早期心肌缺血心肌细胞凋亡的实验研究  

Experimental study on the cardiomyocyte apoptosis in the early myocardial ischemia

作　　者：胡丙杰[1] 陈建国[2] 徐小虎[3] 陈玉川[1] 祝家镇[1] 

机构地区：[1]中山医科大学法医病理学教研室,广东广州510080 [2]广东省人民医院妇产科,广东广州510080 [3]汕头大学医学院法医研究所,广东汕头515031

出　　处：《中国法医学杂志》2001年第2期65-68,共4页Chinese Journal of Forensic Medicine

基　　金：卫生部科研基金! (96 1 12 7);广东省自然科学基金! (980 0 78);卫生部优秀青年科技人才专项基金! (970 0 12 );广东省医药卫生

摘　　要：探讨早期心肌缺血心肌细胞凋亡的意义。采用DNA缺口末端标记法 (TUNEL)对大鼠实验性心肌缺血早期 (6h内 )不同时间缺血损伤区心肌细胞凋亡的情况进行观察。结果 :缺血 1h在缺血区域发现少数散在的凋亡阳性细胞 ,3h达高峰 ,随后下降。正常区域未发现凋亡细胞。缺血边缘区域也在缺血 1h局部开始出现心肌细胞凋亡 ,并随缺血时间延长心肌细胞凋亡指数增加 ,缺血 5h达高峰。表明凋亡是早期缺血性心肌细胞损伤的主要方式 ,心肌凋亡检测可望为早期心肌梗死的死后诊断提供一个灵敏客观的新方法。In order to explore the significance of cardiomyocyte apoptosis in the early myocardial ischemia,the rat model of acute myocardial ischemia was established,and the apoptotic cells were detected in the early phase of ischemia(within 6h)with TUNEL method.The results showed that scanty apoptotic cells could be observed in the ischemic region 1h after ischemia,and reached the peak 3h after ischemia,then decreased.No apoptotic cells were found in the normal region.In the peri ischemic region,apoptotic cells could also be observed 1h after ischemia,and reached the peak 5h after ischemia.It is indicated that apoptosis is the major form of early ischemic myocardial damage,and the detection of apoptotic cardiomyocyte may provide a new sensitive and objective method for the postmortem diagnosis of early myocardial infarction.

关 键 词：心肌缺血 细胞凋亡 DNA缺口末端标记法 

分 类 号：D919[医药卫生—法医学]