慈姑蛋白酶抑制剂(API)基因导入棉花获得转基因植株  

Transgenic Cotton Plant Resulting from Introduction of Arrowhead Proteinase Inhibitor (API) Gene into Cotton

作　　者：黄骏麒[1] 龚蓁蓁[2] 吴敬音[3] 陈松[1] 束春娥[4] 谢伟军[2] 周宝良[1] 沈新莲[1] 

机构地区：[1]江苏省农业科学院经济作物研究所,江苏南京210014 [2]中国科学院上海生物化学研究所,上海200031 [3]江苏省农业科学院农业生物遗传生理研究所,江苏南京210014 [4]江苏省农业科学院植物保护研究所,江苏南京210014

出　　处：《江苏农业学报》2001年第2期65-68,共4页Jiangsu Journal of Agricultural Sciences

基　　金：江苏省"九五"农业科技重大攻关项目 (BE9930 4)

摘　　要：通过花粉管通道技术将慈姑蛋白酶抑制剂API(ArrowheadProteinaseInhibitor)基因转入棉花 ,获得了转基因植株。经对棉铃虫抗虫性鉴定和PCR、RT PCR、Southernblotting分析 。The gene for arrowhead proteinase inhibitor(API) was transferred into cotton plants by the pollen tube pathway method,and transgenic cotton plant was resulted.After tests of resistance to pest insects and analyses of PCR,RT PCR and Southern blotting,the transgenic plants were proved to have expressed the API gene and to be resistant to cotton bollworm.This is the first report so far concerning the pest resistant transgenic cotton with the API gene. [WT5”HZ]

关 键 词：慈姑蛋白酶抑制剂 API基因 棉花 转基因植株 

分 类 号：S562.03[农业科学—作物学]