EPA介导内皮依赖性舒血管效应的组织特异性及其在动脉粥样硬化形成中的特性  

Tissue speciality of endothelium-dependent vascular relaxation mediated by EPA and its relationship with pathophysiological property

作　　者：王莉莉[1] 张雁芳[1] 路新强[1] 汪海[1] 

机构地区：[1]军事医学科学院毒物药物研究所,北京100850

出　　处：《军事医学科学院院刊》2001年第2期119-123,共5页Bulletin of the Academy of Military Medical Sciences

基　　金：国家"973"计划项目!资助 (G1980 5 1112 )

摘　　要：目的 :探讨不同种属动物、不同区域血管内皮细胞乙酰胆碱激活蛋白 (endothelialproteinacetivatedbyacetylcholine ,EPA)介导内皮依赖性舒血管效应的特性及其在高血脂诱发动脉粥样硬化形成过程中的变化特征。方法 :采用离体血管条实验方法 ,以去甲肾上腺素预收缩血管 ,观察乙酰胆碱 (ACh)诱导正常和高血脂动物血管舒张反应 ,并计算EC50 。结果 :在兔主动脉、颈动脉、股动脉、肺动脉、肾动脉 ,猫主动脉、股动脉、肾动脉、肠系膜动脉、冠状动脉 ,ACh均诱导内皮依赖性血管舒张反应 ,但EC50 值不同。兔血管中 ,EPA对于ACh敏感性的顺序依次为肺动脉 >肾动脉 >主动脉 >股动脉 >颈动脉。在猫血管中其顺序为冠状动脉 >主动脉 >股动脉 >肠系膜动脉 >肾动脉。在高血脂诱发动脉粥样硬化兔主动脉、颈动脉及肺动脉上 ,ACh诱导的舒张反应明显降低 ,但在肾动脉和股动脉上无显著变化。结论 :EPA介导的内皮依赖性血管舒张反应广泛存在于猫、兔不同组织的动脉血管上 ,但不同种属动物、不同区域组织血管EPA对于ACh的敏感性不同。在高血脂诱发兔动脉粥样硬化的实验模型上 。Objective:To study the tissue speciality of endothelium dependent vascular relaxation mediated by endothelial protein activated by acetylcholine (EPA)and its relationship with atherosclerosis.Methods:The tension of the isolated artery preparations was recorded using isometric tension method and the isolated endothelium intact vessels derived from normal and hyperlipidemic animal artery.Results:The EPA mediated endothelium dependent vascular relaxation was exhibited in rabbit aorta, renal, femoral, carotid, pulmonary arteries and cat aorta, renal, femoral, mesentery, coronary arteries but with different EC 50 . The sensitivity of EPA to ACh in descending order: pulmonary>renal>aorta>femoral>carotid arteries in rabbits. In cats,coronary>aorta>femoral>mesentary>renal. The endothelium dependent vascular relaxation mediated by EPA in aorta, carotid and pulmonary arteries decreased significantly in hyperlipidemia rabbits.Conclusions:EPA effect existed wildly in testing artery and was of different tissue sensitivity. The decrease in EPA potency was closely related to occurence of atheroclerosis. [

关 键 词：内皮 乙酰胆碱 舒张反应 动脉粥样硬化 EPA 

分 类 号：R392.12[医药卫生—免疫学]