慢性酒精刺激对大鼠下丘脑及伏核内β-内啡肽含量的影响  

The Effect of Chronic Ethanol Exposures and Withdrawal on the Beta-endorphin Content in Rat Nucleus Accumbens and Hypothalamus

作　　者：沈红芬[1] 戈应滨[1] 朱学江[1] 张小虎[1] 王自正[2] 袁孝如[1] 

机构地区：[1]南京医科大学生理学教研室,江苏南京210029 [2]南京医科大学附属南京第一医院放免中心,江苏南京210006

出　　处：《南京医科大学学报（自然科学版）》2001年第4期293-294,324,共3页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省教委自然科学基金资助项目 (0 0KJB3 10 0 0 5 ;99KJD3 10 0 0 5 )

摘　　要：目的 :观察在长期低浓度酒精处理后及撤除酒精给药的过程中 ,大鼠下丘脑及伏核中 β 内啡肽含量的变化 ,探讨酒精对 β 内啡肽系统的影响及其在酒精依赖过程中的作用。 方法 :以 6 % (v/v)的酒精作为大鼠饮水的唯一来源 ,分别取大鼠在饮酒和撤除酒精不同时程的下丘脑及伏核标本 ,用放免法测定每克组织中 β 内啡肽的含量。同时用顶空气相色谱法测定大鼠在饮酒过程中不同时段血中的酒精浓度。结果 :下丘脑及伏核的 β 内啡肽含量在长期 (4周 )饮用酒精后无明显变化 ,在撤除酒精后的 2～ 4天明显升高 ,1周后恢复正常。动物血中酒精浓度在摄入酒精的不同时段无明显差异。结论 :酒精影响下丘脑及伏核中 β 内啡肽的含量是低浓度反复多次刺激的结果 ,撤药后伏核内 β 内啡肽的高含量是动物产生依赖的重要原因。Objective:To investigate the effect of ethanol on the beta-endorphin system and their rat in ethanol dependence, the beta-endorphin content in the nucleus accumbens (Nacc) and hypothalamus of the rats during chronic ethanol expoured withdrawal was measured dependence. Methods:SD rats received drinking water containing 6%(v/v) ethanol for 28 days and withdrawal of this water on the 29th day. The Nacc and hypothalamus of the rats in different groups were obtained and their beta-endorphin content were also measured by radioimmunoassay (RIA). The ethanol concentration in the blood of the rats were also measured by headspace gas chromatography. Results:The beta-endorphin content in Nacc and hypothalamus had no change during the rats′ exposure to the ethanol, but obviously increased in the 2nd and 4th days after ethanol withdrawal. Conclusion:The alteration of the beta-endoephin content in Nacc and hypothalamus may result from the chronic and repeated exposure to low concentration ethanol. The high beta-endorphin content in the Nacc during the ethanol withdrawn may be essential to the athanol dependence in these animals.

关 键 词：Β-内啡肽 伏核 下丘脑 慢性酒精中毒 

分 类 号：R595.6[医药卫生—内科学] R338[医药卫生—临床医学]