内钙拮抗剂TMB-8对过氧化氢所致的培养牛主动脉内皮细胞损伤的保护作用  

Protective Effects of TMB-8 on Hydrogen Peroxide Induced Injury in Bovine Aortic Endothelial Cells

作　　者：何玉红[1] 王斌[1] 张孝清[1] 肖继皋[1] 

机构地区：[1]南京医科大学药理学教研室,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2001年第4期314-315,345,共3页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省教委自然科学基金资助项目 (98KJB3 10 0 0 4;99KJB3 10 0 0 1)

摘　　要：目的 :观察内钙拮抗剂TMB 8对过氧化氢 (H2 O2 )介导的血管内皮细胞损伤的作用。方法 :用 10 μmol/LH2 O2 损伤内皮细胞 ,观察不同浓度TMB 8对损伤内皮细胞形态学的作用 ,检测乳酸脱氢酶 (LDH)释放量、丙二醛 (MDA)生成量、超氧化物歧化酶 (SOD)和谷胱甘肽过氧化物酶 (GSH PX)活性。结果 :TMB 8能减轻内皮细胞形态学的改变 ,明显降低LDH释放 ,抑制MDA产物生成 ,保护SOD和GSH PX的活性。结论 :TMB 8对H2 O2 损伤的内皮细胞具有保护作用。Objective:To evaluate the effects of TMB-8 on the endothelial cell injury by hydrogen peroxide. Methods:Bovine aortic endothelial cells damaged by 10 μmol/L hydrogen peroxide were divided into six groups according to the different concerntrations of TMB-8. The lactate dehydrogenase (LDH), malondia(dehyde (MDA), superoxide dismatase (SOD) and glutathione peroxidase (GSH-Px) were assayed and morphological change of endothelial cell were recorded. Results:The morphological changes of endothelial cell and the release of LDH, MDA and reduction of activities of SOD, GSH-Px, induced by H 2O 2 were inhibited by TMB-8 in the concertration-dependent manner. Conclusion:TMB-8 may protect vascular endothelial cells against hydrogen peroxide.

关 键 词：钙拮抗剂 血管内皮细胞 损伤过氧化氢 保护 

分 类 号：R972[医药卫生—药品] R543[医药卫生—药学]