高三尖杉酯碱对HL-60细胞和QGY7703细胞的作用研究  

Study on HL-60 and QGY7703 Cell Lines Treated by Homoharringtonine

作　　者：金伟[1] 黄青山[1] 侯俊梅[2] 王亚威[3] 周宗祥[1] 应康[1] 谢毅[1] 毛裕民 

机构地区：[1]复旦大学,上海200433 [2]通化市人民医院,吉林通化134000 [3]黑龙江省中西医结合研究所,黑龙江哈尔滨150040 [4]联合基因科技集团有限公司,上海200092

出　　处：《中医药学报》2001年第3期44-45,共2页Acta Chinese Medicine and Pharmacology

摘　　要：采用MTT法和流氏细胞仪技术 ,研究了高三尖杉酯碱对HL -60细胞和QGY770 3细胞的存活率和细胞周期分布影响。结果表明 ,高三尖杉酯碱对HL -60细胞的IC50 是 1 .2 8umol.L-1,对QGY770 3细胞的IC50 是 0 .5 5umol.L-1。高三尖杉酯碱处理HL -60细胞 2 4h后 ,G0 -G1期细胞分布有所增加 ,G2 -M期和S期细胞分布有所降低 ;高三尖杉酯碱处理QGY770 3细胞 2 4h后 ,G2 -M期分布略有降低 ,G0 -G1期细胞分布略有增加 ;高三尖杉酯碱处理HL -60细胞2 4h后 ,G0 -G1期峰前有亚二倍体峰出现 ,说明高三尖杉酯碱在所用剂量下能够诱导HL -60细胞发生凋亡。The cell livability and cell cycle of HL-60 cells and QGY7703 cells treated by homoharringtonine was observed by MTT colorimetry and flow cytometry. We found that the IC 50 of HL-60 cells to homoharringtonine is 1.28 umol.L -1 and the IC 50 of QGY7703 cells to homoharringtonine is 0.55 umol.L -1 . After treated by homoharringtonine for 24 hours, G 0 -G 1 period of HL-60 cells increased, G 2 -M and S period of HL-60 cells decreased; G 2 -M period of QGY7703 cells decreased a little, G 0 -G 1 period of QGY7703 cells increased a little; The appearance of subdiploid peak proved the apoptosis inducing activity of homoharringtonine when it treated HL-60 cells for 24 hours.

关 键 词：高三尖杉酯碱 细胞毒 细胞周期 HL-60细胞 QGY7703细胞 抗癌药 

分 类 号：R979.1[医药卫生—药品]