大鼠肝肠联合移植对移植肠内NOS阳性神经和VIP能神经的影响  

The Changes in NOS Activity and VIP Immunopositive Nerves in the Small Bowel Grafts after Combined Liver-intestine Transplantation in Rat

作　　者：殷光甫[1] 曹福元[1] 孙晓毅[2] 林传友[1] 胡道松[1] 茹立强[1] 王果[2] 

机构地区：[1]华中科技大学同济医学院神经生物学教研室,武汉430030 [2]华中科技大学同济医学院附属同济医院小儿外科,武汉430030

出　　处：《同济医科大学学报》2001年第2期100-102,共3页Acta Universitatis Medicinae Tongji

基　　金：国家自然科学基金资助项目 ( No.3 93 70 0 5 97);卫生部科学基金资助项目 ( 0 73 3 - 5 0 1

摘　　要：应用还原型辅酶 —黄递酶 ( NADPH - d)组织化学和血管活性肠肽 ( VIP)免疫组织化学显色法 ,观察大鼠肝肠联合移植后对移植肠内一氧化氮合酶 ( NOS)阳性神经和 VIP能神经的影响。结果发现 :肝肠联合移植除能提高受体动物的存活率外 ,还能减轻和延迟免疫排斥反应的产生 ,而且移植肠内 NOS和 VIP免疫活性反应较好 ,于中、晚期 ( 7～ 14 d)的移植肠内 ,NOS活性和 VIP免疫活性几乎接近正常水平。提示肠内在神经成分的完好对移植动物的存活有重要的作用。Using NADPH diaphorase histochemical and immunohistochemical techniques, the changes in nitric oxide synthase (NOS) activities and vasoactive intestinal polypeptide (VIP) immunoreactivities in intestinal grafts after combined liver intestine transplantation were observed The results showed that after combined liver intestine transplantation, the survival rate of the recipients was increased, the rejection alleviated or delayed and NOS activities and VIP immunoreactivities in the small bowel grafts exhibited well Within metaphase to late time of transplantation, NOS activities and VIP immunoreactivities was almost normal These results suggested that the integration of enteric nerves was important for the survival of the recipients

关 键 词：大鼠 肝肠联合移植 一氧化氮合酶 血管活性肠肽 VIP能神经 肝移植 肠移植 

分 类 号：R657.3[医药卫生—外科学] R617[医药卫生—临床医学]