红细胞-80℃冷冻保存的实验观察  

Experimental Study of Red Blood Cells after Cold Storage at -80℃

作　　者：余忠清[1] 胡丽华[1] 饶神宗[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院输血科,武汉430022

出　　处：《同济医科大学学报》2001年第2期175-177,共3页Acta Universitatis Medicinae Tongji

摘　　要：观察红细胞 ( RBC)冷冻保存后形态、结构和功能的变化。采用慢速冷冻法。冷冻前后分别检测红细胞平均体积 ( MCV)、红细胞脆性 ( RFT)、全血粘度 ( WBV)、红细胞变形性 ( RBCD)、膜的流动性 ( L FU)、血氧饱和度( SO2 at)、红细胞压积 ( HCT)、红细胞回收率 ( RBCRR)和红细胞免疫功能。结果发现 :红细胞解冻复苏后仍然对低渗盐溶液有较强的抵抗力 ;MCV、 RBCD和 L FU无显著性差异 ( P>0 .0 5 ) ;WBV和 HCT下降 ,RBCRR达 84 .1%( P<0 .0 1) ;SO2 at2 由 3 0 .5 %上升到 98.2 % ;免疫功能各项指标检测均无显著性差异 ( P>0 .0 5 )。提示 :红细胞冷冻保存后其质量不变并可长期进行冷冻保存。The changes in shape, structure and function of red blood cells (RBC) after cold storage were observed by using slow cold storage technique Before and after cold storage, RBC mean corpuscular volume (MCV), RBC fragility (RF), whole blood viscosity (WBV), RBC deformity (RBCD), LFU, SatO 2, hematocrit (HCT), RBC recovery rate (RBCRR) and RBC immune function were measured The result showed that after resuscitation, RBC had vital resistance to hypotonic solution There was no significant difference in MCV, RBCD and LFU ( P >0 05) HCT and WBV were decreased and RBCRR reached 84 1 %(all P <0 01) SatO 2 was increased from 30 5 % to 98 2 % RBC immune function showed no significant difference ( P >0 05) It was suggested that quality of RBC didn't change after cold storage and RBC could be cryopreserved for a long time

关 键 词：红细胞 冷冻保存 形态 结构 功能 

分 类 号：R331.1[医药卫生—人体生理学]