H_2O_2 处理大块牛松质骨后成分分析  

Treatment of massive bovine cancellous bone and analysis of its components

作　　者：罗卓荆[1] 胡蕴玉[1] 刘智广[2] 

机构地区：[1]第四军医大学西京医院全军骨科研究所,陕西西安710033 [2]第四军医大学基础部中心实验室,陕西西安710033

出　　处：《第四军医大学学报》2001年第11期991-994,共4页Journal of the Fourth Military Medical University

基　　金：陕西省自然科学基金!资助项目 (97SM5 7)

摘　　要：目的　研究大块牛松质骨 (MBCB)处理后的各种成分及高浓度 H2 O2 消除异种骨抗原性的机制 .方法　采用干燥失重法及残渣法检测经过系统去抗原处理后的大块异种骨的有机、无机质及水份含量 ;将 2 5块大块新鲜牛松质骨均分为 5组 ,分别用高浓度 H2 O2 浸泡 0 ,2 4,48,72和 96 h,采用氨基酸分析方法研究不同 H2 O2 处理时间的骨块中羟脯氨酸、脯氨酸含量 .结果　经处理后 ,MBCB为色白 ,并呈现出多孔样结构 ,扫描电镜观察 ,松质骨呈清晰的多孔结构 ,孔壁光滑 ;5组检测样品中羟脯氨酸及脯氨酸的含量随着 H2 O2 处理时间的延长均有同步下降的趋势 ,但各组间无显著性差异 (P>0 .0 5 ) ;5组检测样品中 18种氨基酸含量及氨基酸总量随着H2 O2 处理时间的延长亦有同步下降的趋势 ,仅 0 h与 96 h组间 18种氨基酸以及总氨基酸含量有显著性差异 (P<0 .0 5 ) ,其余各组间无显著性差异 (P>0 .0 5 ) ;5组检测样品中羟脯氨酸及脯氨酸在总氨基酸总量所占比例随着 H2 O2 处理时间的延长无明显变化 ,各组间无显著性差异 (P >0 .0 5 ) .结论　大块异种骨经系统去抗原处理后 ,保持了一定的有机及无机质比例 ,既具有良好的力学特性又消除了其抗原性 ,提示高氧化剂引起蛋白质变性是消除大块异种骨抗原性的主要原因 .AIM To analyse the components of antigen eliminated massive bovine cancellous bone (MBCB) and to study the mechanism of high concn H 2O 2 eliminating the antigenicity of xenogenic bone. METHODS Dry weight lost method was applied to measure the organic, mineral, and water components in MBCB; 25 massive flesh bovine cancellous bone blocks were distributed into 5 groups, which were dipped in 300 mL·L -1 concn H 2O 2 for 0, 24, 48, 72, 96 h.The quantities of hydroxyproline and proline in the five groups were measured by amino acid analysis. RESULTS After the antigen eliminating program, MBCB occurred as white colour and porous structure. SEM showed the wall of bone trabecula in the center part of MBCB was quite clear. The quantities of hydroxyproline and proline decreased a little with the time treated in high concn H 2O 2 in the five groups, but no differences between the five groups were found; the same decreasing tendency was found to the quantities of 18 amino acids of the bone blocks in the five groups; difference was only found between 0 h and 96 h group ( P <0.05). The percent of hydroxyproline and proline in amino acids was not found any difference in the five groups ( P >0.05). CONCLUSION The MBCB may contain reasonable rate of organic and mineral material, which is beneficial to keeping the mechanic property of MBCB after its antigen eliminating program. Denaturalization of protein in the condition of oxidation of high concn H 2O 2 would be the major mechanism to eliminate the antigenicity of massive xenogenic bone.

关 键 词：骨移植 氨基酸分析 异种移植 牛松质骨 抗原性 

分 类 号：R687.34[医药卫生—骨科学]