红细胞胰岛素受体微量测定法的建立及初步应用  

The Establishment and Application of A Microassay for Measuring of Rat Erythrocyte Insulin Receptors

作　　者：杨利民[1] 陆钟琦 

机构地区：[1]中国协和医科大学生理教研室

出　　处：《基础医学与临床》1991年第4期59-64,共6页Basic and Clinical Medicine

基　　金：国家自然科学基金

摘　　要：本工作建立了红细胞胰岛素受体微量测定法,反应总体积为0.1ml.只需1.5～2ml血液即可测定。采用4℃温育24小时进行受体分析效果较好。受体测定结果采用平均亲和力剪影处理。同时应用建立的微量法测定了老、中、青年大鼠红细胞胰岛素受体的变化,青年大鼠胰岛素受体数目大于中,老年大鼠,后两者间无显著区别,三种年龄大鼠平均亲和力间无明显差异。A micromethod for measuring erythrocyte insulin receptors is established. Thevolume of blood required for insulin radioreceptor assay is small, from 1.5 to 2 ml. The method approves well when erythrocytes are incubated for 24 hours at 4 ℃. Average affinity profiles are used for calculating parameters of insulin receptor . The micromethod was used in studying the erythrocyte insulin receptors of different age of rats. The results show that young rats have more insulin receptors than middle-aged or old rats. No significant difference is obtained between middle-aged and old rats. The affinities of insulin receptors have little difference among the three age groups.

关 键 词：红细胞 胰岛素 受体 测定 

分 类 号：R446.112[医药卫生—诊断学]