百日咳疫苗血清学效力试验与小鼠保护力试验结果的相关性  

Correlation between Serological Potency and Intracerebral Protection Tests of Pertussis Vaccine in Mice

作　　者：孙述学[1] 宋继萍[1] 陈晓航[1] 王剑虹[1] 梁玉珍[1] 杨淼[1] 陈怀恭[1] 王师真[1] 罗广[1] 孙晓琳[1] 武毅[1] 

机构地区：[1]兰州生物制品研究所,兰州730046

出　　处：《中国生物制品学杂志》2001年第2期107-109,共3页Chinese Journal of Biologicals

摘　　要：目的 了解百日咳疫苗血清学效力试验（ＰＳＰＴ）与小鼠保护力试验（ＭＰＴ）结果的相关性，为该法替代ＭＰＴ积累资料。方法 用不同剂量的ＤＴＰ疫苗免疫小鼠，４ｗ后采血，用ＥＬＩＳＡ测定小鼠血清中抗百日咳菌表面 抗原的总抗体滴度。用平行线法计算疫苗的效力，与ＭＰＴ测定结果进行相关性分析。结果１５批疫苗分别用 ＰＳＰＴ和ＭＰＴ法测定的效力单位相关性显著（ｒ＝ ０．９３５８，ｐ＜０．００１），二者差异无显著意义（Ｐ＞０．０５）。与 ＭＰＴ法相 比，ＰＳＦＦ法有更高的重复性，而且９５％可信限较小。结论ＰＳＰＴ法有望取代ＭＰＴ。Objective To explore the correlation between the serological potency and intracerebral protection tests of pertussis vaccine in mice. Methods Mice were immunized with DTP vaccine at various dosages and bleed 4w later for detecting the titers of total pertussis antibodies by ELISA. The potency of pertussis vaccine was calculated by parallel line method, and the correlation between it and that detected by the intracerebral protection test in mice was analyzed. Results Significant correlation was observed in the potencies of 15 lots of pertussis vaccine detected by the 2 methods(r= 0.9358,p <0.001) .Conclusion Serological potency test might be a substitute for intracerebral protection test in the detection of potency of pertussis vaccine.

关 键 词：百日咳疫苗 血清学 效力试验 ELISA 小鼠 保护力 相关性 

分 类 号：R516.603[医药卫生—内科学] R378.42[医药卫生—临床医学]