外源核酸促核辐射鼠肠腺细胞修复的基因分析  

Analysis of Genes Associated with Exogenous Nucleic Acids Improving the Repair of Intestinal Epithelium After γ Irradiation in Mice

作　　者：崔大祥[1] 曾桂英[2] 王枫[1] 田芙蓉[2] 郭晏海[1] 徐俊荣[1] 闫小君[1] 任东清[1] 苏成芝[1] 

机构地区：[1]第四军医大学全军基因诊断技术研究所,西安710033 [2]第四军医大学放射医学教研室,西安710033

出　　处：《生物化学与生物物理进展》2001年第3期353-357,共5页Progress In Biochemistry and Biophysics

基　　金："2 1 1"工程基金资助 (98X2 0 7)&&

摘　　要：初步探讨外源核酸促电离辐射损伤的小鼠肠腺细胞修复的分子机理 .建立BALB/c小鼠电离辐射后给予外源RNA与生理盐水治疗的 6h、 12h、 2 4h、 4d和 8d的模型 ,采集空肠组织标本后采用消减杂交基础上的LD PCR技术 ,获取与受照小鼠肠腺损伤修复相关的基因克隆 ,对其进行全自动序列分析与GenBank检索 . 6h治疗组同源性较高的序列主要为 :热休克蛋白mRNA、NmimRNA、Dutt1蛋白mRNA、Na ,K ATPaseγ亚单位mRNA等 ;12h治疗组同源性较高的序列为 :碱性磷酸酶mRNA、碱性磷酸酶 2、glkA基因、单链复制着丝粒基因等 ;2 4h治疗组同源性较高的序列为 :抗CEA单链抗体重链可变区基因、抗DNA重链可变区基因、Igkappa链mRNA等 ;4d治疗组同源性较高的序列为 :双特异性磷酸酶、端粒酶相关蛋白家族mRNA、β GABA转运基因、紧张激活蛋白mRNA、FK5 0 6结合蛋白、Ca2 +/Ca2 +调蛋白依赖性基因等 ;8d治疗组同源性较高的序列为 :免疫球蛋白可变区基因、鼠免疫球蛋白DNA、易弯曲肽DNA、tsrglkA基因、修复蛋白A等 .新发现的 18个基因片段递交给GeneBank ,接受号为AF2 40 16 4 AF2 40 181.结果表明 :外源核酸促电离辐射损伤的小鼠肠腺修复的机理可能与一些基因、蛋白质的异常表达有关 ,与免疫系统的作用可能有关 .In order to explore the molecular mechanism of exogenous nucleic acids improving repair of irradiation damaged intestinal epithelium, 45 mice being irradiated by γ ray were treated with 40 μg small intestinal RNA as test group, whose small intestinal specimens were collected respectively at 6 h,12 h,24 h,4 d and 8 d after treatment; 40 mice being irradiated by γ ray were treated with physiological saline as control group, whose small intestinal specimens were collected at the same interval time. Then fragments of genes expressed in test group higher than those in control group, were obtained by using LD PCR based on subtractive hybridization. After that, these gene fragments were cloned into T vectors,and were sequenced. Obtained sequences were searched for GenBank.90 clones associated with repair of irradiation damaged crypt cells were obtained.In test group of 6 h, higher similar sequences mainly were as follows: mRNA for heat shock protein, Nmi mRNA, Dutt1 protein, mRNA for Na,K ATPase gamma subunit,mRNA for surface glycoprotein,Zinc finger type transcript factor,porcine growth hormone releasing hormone gene,Homo sapiens dual specificity phosphatase,etc. In test group of 12 h, higher similar sequences were as follows: alkaline phosphatase mRNA,alkaline phosphatase 2,glkA gene, single stranded replicative centromeric gene,Homo sapiens DMBT1 candidate tumor gene, tRNA Met gene,mouse Ig unrearranged transcribed H chain,thyroxine binding globulin gene,alpha 2 plasmin inhibitor gene, etc;In test group of 24 h, higher similar sequences were as follows: anti CEA ScFv antibody heavy chain vary region,anti DNA antibody Ig heavy chain, mRNA for Ig kappa chain region,anti BONT/A Hc ScFv antibody heavy chain vary region, mRNA for ScFv collagenase heavy chain vary region, AE0199 immunoglobulin heavy chain,mouse Ig gamma chain,Ig rearranged gamma chain mRNA,anti NP antibody IgH,mRNA for arginine/serine kinase,dual specificity phosphatase,family mRNA telomerase associated protein,anti human erB 2 regi

关 键 词：小鼠 电离辐射 细胞修复 表达基因 外源核酸 肠道辐射损伤 

分 类 号：R818.05[医药卫生—放射医学] R818.023[医药卫生—临床医学]