鸭各级卵泡中抑制素α和抑制素/活化素β_A亚基信使RNA表达丰度的研究  

Expression of Inhibin α and Inhibin/Activin βA Subunits in the Developing Follicles of the Duck

作　　者：傅衍[1] 牛冬[1] 阮晖[2] 余旭平[1] 陈功[1] 何国庆[2] 杨培新[1] 

机构地区：[1]浙江大学动物科学学院,杭州310029 [2]浙江大学农业工程与食品科学学院,杭州310029

出　　处：《Acta Genetica Sinica》2001年第6期502-509,共8页

基　　金：国家自然科学基金(批准号:39900106)资助&&

摘　　要：采用非常灵敏的定量竞争 ＲＴ－ＰＣＲ技术对鸭各级卵泡中抑制素α和βＡ亚基的ｍＲＮＡ表达丰度作了研究，发现在各级卵泡中均有此两亚基ｍＲＮＡ的表达，大卵泡中的α亚基表达量要高于βＡ亚基。α亚基ｍＲＮＡ在小黄卵泡（ＳＹＦ）中表达量最高，以其ｍＲＮＡ的平均相对丰度为１．００，则Ｆ１、Ｆ２、Ｆ３、Ｆ４／５、ＬＷＦ（大白卵泡）中α亚基ｍＲＮＡ的平均相对含量分别为： ０.２６±０．０５、０．２８±０．０７、０．５７±０．１２、０．９８±０．０９、０．０２６±０．００６。βＡ亚基 ｍＲＮＡ在Ｆ１中表达量最高，以其ｍＲＮＡ的平均相对丰度为１．００，则 Ｆ２、Ｆ３、Ｆ４／５、ＳＹＥ、ＬＷＦ中βＡ亚基ｍＲＮＡ的平均相对含量分别为：０．２１８±０．０９、０．１１１±０．０３、０．０５８±０．０１１、０．０５３±０．０１３、０．００５±０．００２。结果显示，随着卵泡的增大α亚基表达量降低， βＡ亚基却增加，说明在卵泡发育过程中， α和βＡ亚基的表达是独立调节的，另外，βＡ亚基在Ｆ１中的大量表达，说明Ｆ１可能是形成二聚体抑制素／活化素的主要场所。The very sensitive quantitative competitive reverse transcription-polymerase chain reaction (RT-PCR) was used to investigate the expression of mRNA for the inhibin α and inhibin/activin β_A subunit in the developing ovarian follicles of the duck. The results indicated all follicles showed the expression of mRNA for the inhibin α and inhibin / activin βA The inhibin a subunit mRNA is expressed more abundantly than the βA subunit in the large preovulatory follicles. Competitive RT-PCR revealed that the expression of inhibin a subunit is the highest in small yellow follicles (SYF), the mean relative content for the F1, F2, F3, F4/5 and LWF (large white follicle) was 0.26±0.05, 0.28±0.07, 0.57±0.12, 0.98±0.09 and 0.026±0.006, respectively, compared to a mean relative content of 1.00 for the SYF. The highest level of inhibin/ activin βA mRNA was found in the F1 follicle, the mean relative content for the F2, F3, F4/5 SYF and LWF was 0.218±0.09, 0.111±0.03, 0.058±0.011, 0.053±0.013 and 0.005±0.002, respectively, compared to a mean relative content of 1.00 for the F1 follicle. Our data suggest that the expression of the α subunit is reduced with follicular development whereas βA subunit expression is dramatically enhanced, which indicates the expression of inhibin a and inhibin/ activin βA subunit is differentially regulated during follicular development In addition, the highest level of βA mRNA in F1 follicle indicates the production of dimeric ichbin and / or activin primarily occurred in the largest F1 follicle.

关 键 词：定量竞争RT-PCR 抑制素α亚基 抑制素/活化素βA亚基 卵泡 鸭 MRNA 表达丰度 

分 类 号：Q343[生物学—遗传学]