版纳小耳猪近交系5家系35个微卫星座位的遗传分析  

Genetic Analysis of 35 Microsatellite Loci in 5 Lineages of Xishuangbanna Miniature Pig Inbred Line

作　　者：牛荣[1] 商海涛[1] 魏泓[1] 黄中波[1] 曾养志[2] 

机构地区：[1]第三军医大学实验动物中心,重庆400038 [2]云南农业大学遗传资源研究室,昆明650201

出　　处：《Acta Genetica Sinica》2001年第6期518-526,共9页

基　　金：国家重点基础研究发展规划项目(973)(G2000016106);国家"九五"重点科技攻关项目(96A-23-06-01)

摘　　要：利用３５个微卫星座位对版纳小耳猪近交系５个家系进行了遗传检测。统计各家系的等位基因组成，计算各家系的平均基因纯合率，利用基因频率计算出各家系的平均杂合度及品系间的遗传距离，并进行系统聚类。结果表明各家系的平均基因纯合率均较高，其１５１家系达到８８．７９％；ＰＩＣ（多态信息含量）和平均杂合度均较普通商品猪低；各家系等位基因组成差别较大；各家系间亲缘关系与其近交过程一致。据此认为版纳小耳猪近交系５家系均具有较高的近交程度；其基因多态性和遗传多样性较普通商品猪低；各家系均已构成独立遗传群体。The polymorphism of 35 microsatellites in 5 lineages of Xishuangbanna small-ear miniature pig inbred line (XMI) was analysed. Nmuber of alleles of each lineage was counted, and rates of homozygote for 35 microsatellite loci in 5 lineages were calculated. According to gene frequencies of 35 microsatellites polymorphism information content (PIC) and mean heterozygosity were calculated for each lineage, and genetic distances between these lineage were estimated. The dendrograms were obtained based on genetic distances. The results suggest that rates of homozygote in these lineage are all high, and that is the highest in lineage 151. The results also suggest that polymorphism information content and mean heterozygosity in all the lineages are low. Composition of alleles of each lineage was quite different and the genetic relationship between lineages accorded with the process of inbred line. So it is suggested that the inbreeding degree of 5 lineages of XMI are all high, and the richness of genetic diversity is lower than general commercial pig breeds. It also shows each lineage has been different groups with individual genes.

关 键 词：小型猪 微卫星 亲缘关系 版纳小耳猪 近交系 遗传分析 

分 类 号：S828[农业科学—畜牧学]