黑腹果蝇黑条体突变型的基因定位研究  

Studies of Localization of Black Streak Body Gene in Drosophila melanogaster

作　　者：张菁[1] 曾庆韬[1] 薛小桥[1] 金珊[1] 杨勇[1] 

机构地区：[1]湖北大学生命科学院,武汉430062

出　　处：《Acta Genetica Sinica》2001年第6期527-534,共8页

基　　金：国家自然科学基金(编号:39870413;39930100)&&

摘　　要：黑腹果蝇的体色突变类型常见的有黄体（ｙｅｌｌｏｗ；ｙ）、黑体（ｂｌａｃｋ，ｂ）和黑檀体（ｅｂｏｎｙ，ｅ），分别位于Ｘ染色体，第二染色体和第三染色体上。黑条体突变型是本实验室１９９１年９月从野外采集的黑腹果蝇野生型单雌系后代中发现的自发突变品系，为了探明黑条体突变型是原有黑体突变类型的再现还是新的突变，采用常规杂交方法和互补实验技术对黑腹果蝇黑条体突变型的基因定位进行了探讨。互补测验的结果表明，黑条体与黑檀体杂交的子一代为反式排列的杂合体无互补，表现为突变型，子二代中，由于交换而产生重组类型的顺式排列的杂合体表现为野生型。因此确定黑条体突变基因（ｂｓｒ）与黑檀体突变基因（ｅ）是等位的，位于第三染色体的９３Ｄ２区，但分别位于不同的位点上，属于同一顺反子的新的点突变。同时对于各体色基因间的相互作用及遗传传递方式的进行了讨论。Mutation type of body color shows frequently yellow (y), black (b) and ebony (e) in Drosophila melanogaster and locates on X, second and third chromosom respectively. A mutant of black streak (bsr) is a spontaneous mutation strain building in the laboratory in 1991. Whether this mutation strain is rediscovered on the primary style or not. We have discussed this problem with general cross breeding and complementation test. The result of complementation test indicated that it is a mutant in first filial generation with trans heterozygote and that it is few wild in second filial generation with cis teterozygote due to crossing over in crossing bsr and e. But we believe that bsr and e is located same loci (93D2) but not the same sites. This report discussed for correlation inter-gene body color and ruleless of heredity passing down for black streak body.

关 键 词：黑条体 黑檀体 突变型 基因定位 顺反子 位点 互补测验 黑腹果蝇 

分 类 号：Q343[生物学—遗传学]