Ogura细胞质雄性不育紫菜苔×萝卜属间杂种F_1的获得及细胞遗传学研究  

Production and Cytogenctics of Intergeneric Hybrids Between Ogura CMS Brassica campestris var. purpuraria and Raphanus sativus

作　　者：黄邦全[1] 常玲[1] 居超民[1] 陈建国[1] 

机构地区：[1]湖北大学生命科学学院,武汉430062

出　　处：《Acta Genetica Sinica》2001年第6期556-561,共6页

基　　金：武汉市青年科技晨光计划;湖北省教育厅青年基金资助项目&&

摘　　要：以Ｏｇｕｒａ细胞质雄性不育紫菜苔（ＡＡ，２ｎ＝２０）为母本，以不同萝卜品种（ＲＲ，２ｎ＝１８）为父本进行杂交，获得了大量的属间杂种。杂种Ｆ１幼苗在低温下子叶及真叶均不缺绿。以红萝卜为父本获得的杂种Ｆ１植株叶柄、叶脉呈紫红色；以白萝卜为父本获得的杂种Ｆ１植株叶柄、叶脉不呈紫红色。所有的杂种Ｆ１植株都开白花，蜜腺正常，雄配子高度不育，但是雌配子具有部分育性。杂种Ｆ１花粉母细胞的染色体数目为预期的２ｎ＝１９，染色体平均配对构型为１５．５３Ⅰ＋１．３４Ⅱ＋０．２５Ⅲ＋０．０１Ⅳ，多数染色体以单价体的形式存在，但也有一些二价体、三价体甚至四价体，最多达到６Ⅰ＋３Ⅲ＋１Ⅳ，参加配对的染色体数达到１３条，表明Ａ染色体组和Ｒ染色体组具有一定的同源性。对该杂种的遗传及育种意义进行了讨论。Crosses between Ogura CMS Brassica campestris var. purpuraria (AA, 2n=20) and Raphanus sativus (RR, 2n=18) were made and many intergeneric hybrids were produced. The F1 seedlings did not show chlorosis at low temperature. When red Raphanus sativus varieties were used as male parent the leaf petiole and leaf vein of F1 plants were purple, and when white Raphanus sativus varieties were used as male parent the leaf petiole and leaf vein of F1 plants were not purple. All the F1 plants had white flowers and normal honey glands. Male gametes of the F1 were highly sterile and female garnetes of the F1 were partly fertile. Cytological studies indicated that chromosome number of the F1 was 2n=19 as expected, the mean chromosome pairing pattern was 15.53Ⅰ+1.34Ⅱ+0.25Ⅲ+0.01Ⅳ. Most chromosomes exsistet as univalents, but there also exsisted some bivalents, trivalents and even tetravalents, suggesting that chromosome set A was partly homologous with chromosome set R

关 键 词：Ogura细胞质雄性不育 紫菜苔 萝卜 属间杂种 细胞遗传学 

分 类 号：Q943[生物学—植物学]