转基因棉花的Bt基因流  

Bt Gene Flow of Transgeic Cotton

作　　者：沈法富[1] 于元杰[1] 张学坤 毕建杰[1] 尹承佾[1] 

机构地区：[1]山东农业大学植物遗传工程实验室,泰安271018 [2]山东省棉花研究中心,济南250100

出　　处：《Acta Genetica Sinica》2001年第6期562-567,共6页

基　　金：山东省重点攻关项目(981154511)&&

摘　　要：通过两年实验检测转基因陆地棉品种间和海岛棉与陆地棉种间的Ｂｔ基因流。将转基因陆地棉国抗１２号种植在１２ｍ×１２ｍ的样方内，周围分别种植非转基因陆地棉品种中棉所１２号和海岛棉品种新海１３号，在离转基因棉花不同距离选取样点，采集非转基因棉花种子，利用标记选择基因、Ｄｏｔ－ＥＬＩＳＡ和ＰＣＲ扩增检测Ｂｔ基因流。结果表明：在０～６ｍ内陆地棉品种间显示较高频率的基因流，随着距离的增加Ｂｔ基因流降低，最大可达３６ｍ；提高样方内转基因棉花纯度，仅增加０～３ｍ内基因流，对较远距离基因流无影响。海陆种间Ｂｔ基因流在０～６ｍ内比陆地棉品种间低，但Ｂｔ基因流随距离增加下降幅度小，最远达７２ｍ。因此，在小规模转基因棉花环境释放实验，可选用同种不同品种棉花作为缓冲隔离带，并讨论了棉花转基因逃离至我国棉属种类的可能性。This study was carried out to determine the gene flow of transgenic cotton under Chinese ecological environment. Transgenic cotton GK-12 containing the marker gene NPTⅡ and Bt gene was planted in the 6×6m2 plot, non-transgenic cotton CCRC 12 and Xinmian 13 were planted respectively around them. At varying distances from transgenic cotton, seeds produced by the non-transgenic cotton were collected and screened for marker gene and Bt gene using kanamycine sulphate and Dot-ELISA method. PCR technique was also used in some seeds to screen Bt gene. The result indicated that gene flow was found to be high at 0-6 m, and to decrease with distances; however gene flow occurred up to distance of 36 m from the transgenic cotton plot. Bt gene flow at 3-6 m increased with increasing the diversity of transgenic cotton in the plot but gene flow increased little at long distance. The gene flow between species was lower than between cultivars at 0-6 m, and occurred at the distance of 72 m from transgenic plot. 72 m buffer zones would serve to limit gene flow of transgenic cotton from small-scale field test. The possibility of escapes of engineered gene to wild relatives of cotton species was also discussed.

关 键 词：转基因棉花 Bt基因流 风险评价 陆地棉 海岛棉 

分 类 号：Q943[生物学—植物学]