不同戊肝抗原检测抗-HEVIgM反应性研究  

Immunoreactivity Studies of Various HEV Antigen with Anti-HEV IgM of the Sear from Patients Infected with Hepatitis E Virus

作　　者：戎广亚[1] 周继文[1] 雷厉[1] 赵桂兰[1] 孙杰[1] 

机构地区：[1]北京解放军第302医院临床诊断试剂研究中心,北京100039

出　　处：《中国病毒学》2001年第2期128-130,共3页Virologica Sinica

摘　　要：目的　比较不同戊肝抗原检测抗 -HEVIgM反应性。方法用HEVE30、E42、E33合成肽和HEVORF 2重组抗原建立酶免疫试验 (EIA)检测肝病患者和健康人群中抗 HEVIgM。结果 6 0份抗 HEV阳性血清中 ,用E30、E42、E33及重组抗原包被检测抗 HEVIgM ,阳性率分别为 76 .6 % ,2 6 .6 % ,18.3 % ,6 6 .7%。用E30抗原进一步检测戊肝急性期及恢复期血清 ,抗HEVIgM阳性率为 90 %及 3 .3 %。结论以HEVE30为抗原的EIA特异性强、灵敏度高 ,是戊型肝炎早期诊断实用可靠的方法。Objective To compare the Immunoreactivity of various HEV Antigen with Anti HEV IgM. Methods Solid phase enzyme immunoassay( EIA ) was developed for detecting anti HEV IgM by using synthetic peptides E30, E42, E33, and recombinant antigen from HEV ORF 2. Results Of 60 anti HEV positive sera by using E30, E42, E33 and recombinant antigen as coating antigen, Anti HEV IgM positive rates were 76.7%, 26.6%, 18.3% and 66.7% respectively. In Acute phase and convalescence phase sera of the patients with Hepatitis E, Anti HEV IgM positive rate was 90% and 3.3% respectively. Conclusions The HEV E30 based EIA will be very useful in the early diagnosis of Hepatitis E.

关 键 词：戊型肝炎病毒 合成肽 重组抗原 

分 类 号：R446.6[医药卫生—诊断学]