丙型肝炎患者血浆和外周血单个核细胞同步检测HCV-RNA和HGV-RNA  

The Synchronous Detection on HCV-RNA and HGV-RNA in Plasma and in Peripheral Blood Mononuclear Cells of Patients with Hepatitis C

作　　者：李淑莉[1] 曾令兰[1] 罗端德[1] 刘薇[1] 郭劲松[1] 杨小铭[1] 

机构地区：[1]同济医科大学附属协和医院传染科,湖北武汉430022

出　　处：《中国病毒学》2001年第2期188-189,共2页Virologica Sinica

摘　　要：To understand the incidence of hepatitis C and hepatitis G coinfection, and positive rate of HCV RNA or HGV RNA in plasma and PBMC, HCV RNA and HGV RNA in plasma and in peripheral blood mononuclear cells(PBMC) of the 40 patients were amplified with RT PCR. There were 6 and 8 HGV RNA positive cases in plasma and PBMC, respectively. And there were 5 HGV RNA positive cases both in plasma and PBMC.At the same time, there were 5,6,3 both HCV RNA and HGV RNA positive cases in plasma, in PBMC and in both plasma and PBMC,respectively. It accounted for 13%,15% and 8%, respectively. The HCV RNA and HGV RNA positive incidence of PBMC was higher than that of plasma. The incidence of HCV RNA and HGV RNA coinfection was similar to European, American and Japanese. The synchronous detection has an important signficance in avoiding leaked diagnosis.To understand the incidence of hepatitis C and hepatitis G coinfection, and positive rate of HCV RNA or HGV RNA in plasma and PBMC, HCV RNA and HGV RNA in plasma and in peripheral blood mononuclear cells(PBMC) of the 40 patients were amplified with RT PCR. There were 6 and 8 HGV RNA positive cases in plasma and PBMC, respectively. And there were 5 HGV RNA positive cases both in plasma and PBMC.At the same time, there were 5,6,3 both HCV RNA and HGV RNA positive cases in plasma, in PBMC and in both plasma and PBMC,respectively. It accounted for 13%,15% and 8%, respectively. The HCV RNA and HGV RNA positive incidence of PBMC was higher than that of plasma. The incidence of HCV RNA and HGV RNA coinfection was similar to European, American and Japanese. The synchronous detection has an important signficance in avoiding leaked diagnosis.

关 键 词：丙型肝炎病毒 HCV 庚型肝炎病毒 HGV 重叠感染 血浆 单个核细胞 

分 类 号：R512.63[医药卫生—内科学] R446.5[医药卫生—临床医学]